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KDM3B Recombinant Rabbit Monoclonal Antibody [1A5]

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Specification

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Catalog# HA721169

KDM3B Recombinant Rabbit Monoclonal Antibody [1A5]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

KDM3B Recombinant Rabbit Monoclonal Antibody [1A5]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human KDM3B aa 251-500.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 191 kDa

Positive Control

Hela cell lysate, NIH/3T3 cell lysate, A431, Hela, human testis tissue, human fallopian tube tissue.

Conjugation

unconjugated

Clone Number

1A5

RRID

AB_3072291

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:400-1:2,000

Target

Function

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. Its gene is located in the 5q region of the genome which is deleted in del(5q) interstitial deletion, a frequent deletion found in myeloid leukemias and myelodysplasias, suggesting that it may be a good candidate for the del(5q) tumor suppressor gene. A multitude of publications have implicated JMJD1A as a driver of tumorigenesis in a variety of different cancers. Less evidence hints at JMJD1B and JMJD1C as potential oncogenic proteins. And there is persuasive data to suggest that JMJD1B is a tumor suppressor, especially in AML, and JMJD1A and JMJD1C may also be tumor suppressive in germ cell tumors. Tissue specificity: Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.

Background References

1. Yuan Sui, Ruicai Gu, Ralf Janknecht. Crucial Functions of the JMJD1/KDM3 Epigenetic Regulators in Cancer. Mol Cancer Res. 2021 Jan; 19(1): 3–13.

2. Yamane K., Toumazou C., Tsukada Y.I., Erdjument-Bromage H., Tempst P., Wong J., Zhang Y. JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. Cell 125:483-495(2006).

Subcellular Location

Nucleus

UNIPROT

SwissProt: Q7LBC6 Human

SwissProt: Q6ZPY7 Mouse

Synonyms

5qNCA antibody

C5orf7 antibody

JHDM2B antibody

JmjC domain containing histone demethylation protein 2B antibody

JmjC domain-containing histone demethylation protein 2B antibody

jmjd1b antibody

Jumonji domain containing 1B antibody

Jumonji domain containing protein 1B antibody

Jumonji domain-containing protein 1B antibody

KDM3B antibody

Expand

Images

  • Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate (30 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (25 µg/Lane)<br /><br />Predicted band size: 191 kDa<br />Observed band size: 191 kDa<br /><br />Exposure time: 30 seconds;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (HA721169) at 1/1,000 dilution.

    Lane 1: Hela cell lysate (30 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (25 µg/Lane)

    Predicted band size: 191 kDa
    Observed band size: 191 kDa

    Exposure time: 30 seconds;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721169) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A431 cells labeling KDM3B with Rabbit anti-KDM3B antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of A431 cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA721169) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA721169) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of Hela cells labeling KDM3B with Rabbit anti-KDM3B antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA721169) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA721169) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3B antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3B antibody (HA721169) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721169) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-KDM3B antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721169" style="font-weight: bold;text-decoration: underline;">HA721169</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-KDM3B antibody (HA721169) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721169) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation