KDM3B Recombinant Rabbit Monoclonal Antibody [1A8]
Catalog# HA721170
KDM3B Recombinant Rabbit Monoclonal Antibody [1A8]
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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HA750509
不含抗保成分
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unconjugated
Overview
Product Name
KDM3B Recombinant Rabbit Monoclonal Antibody [1A8]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human KDM3B aa 251-500.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Cell, FC
Molecular Weight
Predicted band size: 191 kDa
Positive Control
Hela cell lysate, NIH/3T3 cell lysate, Hela, human testis tissue, human fallopian tube tissue.
Conjugation
unconjugated
Clone Number
1A8
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:400-1:2,000
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FC
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1:500-1:1,000
Target
Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. Its gene is located in the 5q region of the genome which is deleted in del(5q) interstitial deletion, a frequent deletion found in myeloid leukemias and myelodysplasias, suggesting that it may be a good candidate for the del(5q) tumor suppressor gene. A multitude of publications have implicated JMJD1A as a driver of tumorigenesis in a variety of different cancers. Less evidence hints at JMJD1B and JMJD1C as potential oncogenic proteins. And there is persuasive data to suggest that JMJD1B is a tumor suppressor, especially in AML, and JMJD1A and JMJD1C may also be tumor suppressive in germ cell tumors. Tissue specificity: Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
Background References
1. Yuan Sui, Ruicai Gu, Ralf Janknecht. Crucial Functions of the JMJD1/KDM3 Epigenetic Regulators in Cancer. Mol Cancer Res. 2021 Jan; 19(1): 3–13.
2. Yamane K., Toumazou C., Tsukada Y.I., Erdjument-Bromage H., Tempst P., Wong J., Zhang Y. JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. Cell 125:483-495(2006).
Subcellular Location
Nucleus
Synonyms
5qNCA antibody
C5orf7 antibody
JHDM2B antibody
JmjC domain containing histone demethylation protein 2B antibody
JmjC domain-containing histone demethylation protein 2B antibody
jmjd1b antibody
Jumonji domain containing 1B antibody
Jumonji domain containing protein 1B antibody
Jumonji domain-containing protein 1B antibody
KDM3B antibody
ExpandImages
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Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (HA721170) at 1/1,000 dilution.
Lane 1: Hela cell lysate (25 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Predicted band size: 191 kDa
Observed band size: 191 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721170) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA721170) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA721170) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3B antibody (HA721170) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721170) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-KDM3B antibody (HA721170) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721170) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Hela cells labeling KDM3B.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721170, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"