6X His-tag, AlpHcAbs Rabbit Alpaca Monoclonal Antibody (HA721172)
Catalog# HA721172
Alpaca Monoclonal to 6X His-tag, AlpHcAbs Rabbit
-
WB
-
IP
-
ELISA
Lane 1: C-terminal His-tag fusion protein lysate
Lane 2: N-terminal His-tag fusion protein lysate
Lane 3/4/5/6: Mid His-tag fusion protein lysate
Lysates/proteins at 50 ng/Lane.
Observed band size: 30/45/50 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721172) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Alpaca monoclonal primary
Product Name
6X His-tag, AlpHcAbs Rabbit Alpaca Monoclonal Antibody (HA721172)
Immunogen
6*his tag fusion protein.
Host
Alpaca
Positive Control
C-terminal His-tag fusion protein lysate, N-terminal His-tag fusion protein lysate, Mid His-tag fusion protein lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
APPLICATION DILUTION
-
WB
-
1:10,000-1:50,000 (C-His)
-
WB
-
1:500-1:10,000 (N-His)
-
WB
-
1:10,000-1:50,000 (Mid-His)
-
IP
-
1:100-1:500
-
ELISA
-
1:10,000-1:50,000
PROPERTIES
Form
Liquid
Storage Condition
Store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS pH=7.5
Concentration
1ug/ul
Purification
Protein A affinity purified.
TARGET
PROTEIN NAME
6X His-tag, AlpHcAbs Rabbit
SYNONYMS
6 His epitope tag antibody;Hexa His tag antibody;HHHHHH epitope tag antibody;HHHHHH tag antibody;His tag antibody;Polyhistidine Tag antibody
FUNCTION
His-Tag Antibody is a high quality monoclonal His-Tag antibody (also designated 6X His-Tag antibody or His-Probe antibody) suitable for the detection of the His-Tag protein. His-Tag Antibody is available as both the non-conjugated anti-His-Tag antibody form, as well as multiple conjugated forms of anti-His-Tag antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. Anti-His tag, AlpHcAbs Rabbit antibody is monovalentrecom mbinant single domain antibody fused to rabbit 1gG Fc,and Anti-His tag AlpHcAbs Rabbit antibody detects the His tag selectively,no re eactivity with other proteins.
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