EZH1 Recombinant Rabbit Monoclonal Antibody [PS01-09] (HA721187)
Catalog# HA721187
Rabbit Monoclonal to EZH1
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of EZH1 on different lysates with Rabbit anti-EZH1 antibody (HA721187) at 1/1,000 dilution.
Lane 1: HCT116 cell lysate (20 µg/Lane)
Lane 2: K562 cell lysate (20 µg/Lane)
Lane 3: PANC-1 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (17 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 85/110 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721187) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT116 cell lysate (20 µg/Lane)
Lane 2: K562 cell lysate (20 µg/Lane)
Lane 3: PANC-1 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (17 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 85/110 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721187) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
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