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ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44]

Usd: 385

Specification

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Catalog# HA721194

ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44]

Application

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ErbB3 aa 650-750 (internal sequence).

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 148 kDa

Positive Control

Human small intestine tissue.

Conjugation

unconjugated

Clone Number

PD00-44

RRID

AB_3072314

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:200

Target

Function

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Background References

1. Eliseev IE et al. Targeting ErbB3 Receptor in Cancer with Inhibitory Antibodies from Llama. Biomedicines. 2021 Aug

2. Kiavue N et al. ERBB3 mutations in cancer: biological aspects, prevalence and therapeutics. Oncogene. 2020 Jan

Subcellular Location

Cell membrane, Secreted.

UNIPROT

SwissProt: P21860 Human

Synonyms

c erbB 3 antibody

c erbB3 antibody

Erb b2 receptor tyrosine kinase 3 antibody

ErbB 3 antibody

ERBB3 antibody

ERBB3 protein antibody

erbB3 S antibody

ERBB3_HUMAN antibody

Glial growth factor receptor antibody

HER 3 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ErbB3/HER3 antibody (<a href="/products/HA721194" style="font-weight: bold;text-decoration: underline;">HA721194</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721194" style="font-weight: bold;text-decoration: underline;">HA721194</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721194) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ErbB3/HER3 antibody (<a href="/products/HA721194" style="font-weight: bold;text-decoration: underline;">HA721194</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721194" style="font-weight: bold;text-decoration: underline;">HA721194</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721194) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling ErbB3/HER3 with Rabbit anti-ErbB3/HER3 antibody (<a href="/products/HA721194" style="font-weight: bold;text-decoration: underline;">HA721194</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721194" style="font-weight: bold;text-decoration: underline;">HA721194</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling ErbB3/HER3 with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721194, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Phospho-ErbB3/HER3 (Y1289) Recombinant Rabbit Monoclonal Antibody [JE51-25]

Application: WB,IF-Cell

Reactivity: Human

Conjugate: unconjugated