IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89]
Catalog# HA721205
IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89]
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human IGJ aa 50-150 (internal sequence).
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 18 kDa
Positive Control
Daudi cell lysate, Ramos cell lysate, Mouse liver tissue lysate, Daudi, human tonsil tissue.
Conjugation
unconjugated
Clone Number
PD00-89
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IF-Cell
-
1:2,000
-
IHC-P
-
1:2,000
-
FC
-
1:500-1:1,000
Target
Function
A J chain is a protein component of the antibodies IgM and IgA. It is a 137 residue polypeptide, encoded by the IGJ gene. The J Chain is required for IgM or IgA to be secreted into mucosa. As part of a polymeric immunoglobulin (pIg), the J-chain is essential for binding of pIg to the pIgR, which forms the secretory component upon excretion of the secretory pIg by epithelial cells. This binding facilitates transport of the J-chain positive pIg molecules from the basal to the apical sides of epithelial cells. Because IgM and IgA are the only two types of antibody that polymerize, initial hypotheses stated that J chain was required for polymerization. However, it was subsequently found that IgM is able to polymerize in the absence of J chain as both a pentamer and a hexamer, however, both of these exist to lesser numbers in organisms lacking J chains. In such cases, there are also fewer IgA dimers. The J-chain also plays a role in the activation of complement. J-chain negative IgM hexamers are 15-20 times more effective at activating complement than J-chain positive IgM pentamers. A consequence of this lack of complement activation is it allows J-chain positive pIgM to bind antigens without causing excessive damage to epithelial membranes through complement activation.
Background References
1. Kim JY et al. The HSS3/4 enhancer of Crlz1-IgJ locus is another target of EBF in the pre-B cell stage of B cell development. Immunol Lett. 2006 Sep
2. Cho SJ et al. A Stat5-overlapping site is critical for the IgJ enhancer activity in the plasma cells and bound by a ubiquitous protein. Biochem Biophys Res Commun. 2005 Dec
Subcellular Location
Secreted.
Synonyms
IGCJ antibody
IGJ_HUMAN antibody
Immunoglobulin J chain antibody
Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody
immunoglobulin joining chain antibody
JCH antibody
JCHAIN antibody
Joining chain of multimeric IgA and IgM antibody
Images
-
☑ Relative expression (RE)
Western blot analysis of IGJ on different lysates with Rabbit anti-IGJ antibody (HA721205) at 1/5,000 dilution.
Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (negative) (20 µg/Lane)
Lane 3: Ramos cell lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Predicted band size: 18 kDa
Observed band size: 25 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721205) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of Daudi (positive) and HeLa (negative) labeling IGJ with Rabbit anti-IGJ antibody (HA721205) at 1/2,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGJ antibody (HA721205) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IGJ antibody (HA721205) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721205) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Daudi cells labeling IGJ.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721205, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"