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NAPSIN A Recombinant Rabbit Monoclonal Antibody [PD01-30]

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Specification

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Catalog# HA721218

NAPSIN A Recombinant Rabbit Monoclonal Antibody [PD01-30]

Application

  • IHC-P

  • IF-Tissue

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NAPSIN A Recombinant Rabbit Monoclonal Antibody [PD01-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant fragment within Human NAPSIN A aa 1-150.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue, WB

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Human lung carcinoma tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

PD01-30

RRID

AB_3072338

Product Features

Form

Liquid

Concentration

1 mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:1,000

  • WB

  • 1:2,000

Target

Function

This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. Napsin A plays a role in pneumocyte surfactant processing. In normal tissue, Anti-Napsin A specifically labels type II pneumocytes in adult lung and epithelial cells in kidney tissues. In abnormal tissues, Napsin A is strongly positive in over 80% of primary lung adenocarcinomas and 79% of renal cell carcinoma by immunohistochemistry. Napsin A is a useful marker for lung adenocarcinoma. The combined use of Napsin A and thyroid transcription factor-1 (TTF-1) improves the sensitivity and specificity for identification of pulmonary adenocarcinoma.

Background References

1. Rawlings ND and Salvesen GS. Handbook of Proteolytic Enzymes Volume 1. 3rd Edition. Academic Press. 2013; p.69-71.

2. Mukhopadhyay, Sanjay, and Anna-Luise A Katzenstein. "Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1, napsin A, p63, and CK5/6." The American journal of surgical pathology vol. 35,1 (2011): 15-25.

Subcellular Location

Secreted.

UNIPROT

SwissProt: O96009 Human

Synonyms

Asp 4 antibody

ASP4 antibody

Aspartyl protease 4 antibody

KAP antibody

Kdap antibody

Kidney derived aspartic protease like protein antibody

NAP1 antibody

NAPA antibody

Napsa antibody

NAPSA_HUMAN antibody

Expand

Images

  • Western blot analysis of NAPSIN A on different lysates with Rabbit anti-NAPSIN A antibody (<a href="/products/HA721218" style="font-weight: bold;text-decoration: underline;">HA721218</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 (Human embryonic kidney cell)<br />Lane 2: A549 (Human lung adenocarcinoma cell)<br />Lane 3: K-562 (Human chronic myelogenous leukemia cell)<br />Lane 4: HUVEC (Human umbilical vein endothelial cell)<br />Lane 5: Mouse lung<br /><br />cell lysate 20 µg/Lane.<br />tissue lysate 40 µg/Lane.<br /><br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721218" style="font-weight: bold;text-decoration: underline;">HA721218</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 45.4 kDa<br />Observed band size: 45.4 kDa

    Western blot analysis of NAPSIN A on different lysates with Rabbit anti-NAPSIN A antibody (HA721218) at 1/2,000 dilution.

    Lane 1: HEK-293 (Human embryonic kidney cell)
    Lane 2: A549 (Human lung adenocarcinoma cell)
    Lane 3: K-562 (Human chronic myelogenous leukemia cell)
    Lane 4: HUVEC (Human umbilical vein endothelial cell)
    Lane 5: Mouse lung

    cell lysate 20 µg/Lane.
    tissue lysate 40 µg/Lane.

    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721218, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 45.4 kDa
    Observed band size: 45.4 kDa

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Lung carcinoma<br />Sample: Paraffin-embedded section<br /><br />Primary antibody dilution: 1/2,000<br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes<br />Platform: Leica Biosystems BOND&reg; RX

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Lung carcinoma
    Sample: Paraffin-embedded section

    Primary antibody dilution: 1/2,000
    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes
    Platform: Leica Biosystems BOND® RX

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NAPSIN A antibody (<a href="/products/HA721218" style="font-weight: bold;text-decoration: underline;">HA721218</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721218" style="font-weight: bold;text-decoration: underline;">HA721218</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NAPSIN A antibody (HA721218) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721218) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-NAPSIN A antibody (<a href="/products/HA721218" style="font-weight: bold;text-decoration: underline;">HA721218</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721218" style="font-weight: bold;text-decoration: underline;">HA721218</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-NAPSIN A antibody (HA721218) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721218) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: Lung carcinoma<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: Lung carcinoma

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"