SPINK1 Recombinant Rabbit Monoclonal Antibody [PD00-93]
Catalog# HA721221
SPINK1 Recombinant Rabbit Monoclonal Antibody [PD00-93]
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IHC-P
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Human
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unconjugated
Overview
Product Name
SPINK1 Recombinant Rabbit Monoclonal Antibody [PD00-93]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant full length protein corresponding to Human SPINK1/P12 aa 1 to the C-terminus.
Species Reactivity
Human
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 9 kDa
Positive Control
Human pancreas tissue.
Conjugation
unconjugated
Clone Number
PD00-93
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:2,000
Target
Function
Pancreatic secretory trypsin inhibitor (PSTI) also known as serine protease inhibitor Kazal-type 1 (SPINK1) or tumor-associated trypsin inhibitor (TATI) is a protein that in humans is encoded by the SPINK1 gene. Mutations in SPINK1 has been associated with hereditary pancreatitis and tropical pancreatitis. Trypsinogen is normally created and stored an inactive zymogen of trypsin in the pancreas, but occasionally will autoactivate itself. PSTI serves to cleave prematurely activated trypsin to prevent the enzyme from causing cellular damage to the organ. Without the function of PSTI, the pancreas is subject to repeated episodes of damage. p.N34S mutation leads to idiopathic pancreatitis (for those reading zarovizsga). It has also been associated with prostate cancer.
Background References
1. Ru N et al. SPINK1 mutations and risk of pancreatic cancer in a Chinese cohort. Pancreatology. 2021 Aug
2. Wang F et al. Performance of SPINK1 and SPINK1-based diagnostic model in detection of hepatocellular carcinoma. J Clin Lab Anal. 2021 Nov
Subcellular Location
Secreted.
UNIPROT
Synonyms
ISK1_HUMAN antibody
Pancreatic secretory trypsin inhibitor antibody
Pancreatic serine protease inhibitor Kazal antibody
PCTT antibody
PSTI antibody
Serine protease inhibitor Kazal type 1 antibody
Serine protease inhibitor Kazal-type 1 antibody
Spink 3 antibody
SPINK1 antibody
Spink3 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SPINK1 antibody (HA721221) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721221) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"