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hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-02]

Usd: 385

Specification

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Catalog# HA721226

hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-02]

Application

  • WB

  • FC

Reactivity

  • Human

BSA and Azide free
Predicted reactivity

  • Mouse

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within C terminal human SET1A.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 186 kDa

Positive Control

293T cell lysates, 293T, HepG2.

Conjugation

unconjugated

Clone Number

PH00-02

RRID

AB_3072346

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place. Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis. Required for H3K4me1 mark at stalled replication forks. Mediates FANCD2-dependent nucleosome remodeling and RAD51 nucleofilaments stabilization at reversed forks, protecting them from nucleolytic degradation. Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated.

Background References

1. Kummeling J., et al. Characterization of SETD1A haploinsufficiency in humans and Drosophila defines a novel neurodevelopmental syndrome. Mol. Psychiatry 26:2013-2024 (2021)

2. Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell- proliferation factor HCF-1. Genes Dev. 17:896-911 (2003)

Subcellular Location

Chromosome, Nucleus

UNIPROT

SwissProt: O15047 Human

SwissProt: E9PYH6 Mouse

SwissProt: A0A8I6AS32 Rat

Synonyms

Histone-lysine N-methyltransferase SETD1A antibody

hSET1A antibody

KMT2F antibody

Lysine N methyltransferase 2F antibody

Lysine N-methyltransferase 2F antibody

SET domain containing 1A antibody

SET domain containing protein 1A antibody

SET domain-containing protein 1A antibody

SET1 antibody

Set1 Ash2 histone methyltransferase complex subunit SET1 antibody

Expand

Images

  • Flow cytometric analysis of 293T cells labeling hSET1/SET1.<br /><br />Cells were fixed and permeabilized.Then stained with the primary antibody (<a href="/products/HA721226" style="font-weight: bold;text-decoration: underline;">HA721226</a>, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of 293T cells labeling hSET1/SET1.

    Cells were fixed and permeabilized.Then stained with the primary antibody (HA721226, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of HepG2 cells labeling hSET1/SET1.<br /><br />Cells were fixed and permeabilized.Then stained with the primary antibody (<a href="/products/HA721226" style="font-weight: bold;text-decoration: underline;">HA721226</a>, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling hSET1/SET1.

    Cells were fixed and permeabilized.Then stained with the primary antibody (HA721226, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (<a href="/products/HA721226" style="font-weight: bold;text-decoration: underline;">HA721226</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: HEK 293T cell lysate<br />Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate<br />Lane 4: HepG2 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 1 minute 30 seconds<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721226" style="font-weight: bold;text-decoration: underline;">HA721226</a>, 1/1,000 in primary antibody, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 186.0 kDa<br />Observed band size: 250~300 kDa

    Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (HA721226) at 1/1,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: HEK 293T cell lysate
    Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 4: HepG2 cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 1 minute 30 seconds


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721226, 1/1,000 in primary antibody, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 186.0 kDa
    Observed band size: 250~300 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"