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Kir2.1 Recombinant Rabbit Monoclonal Antibody [JE54-56]

Usd: 385

Specification

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Catalog# HA721227

Kir2.1 Recombinant Rabbit Monoclonal Antibody [JE54-56]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Kir2.1 Recombinant Rabbit Monoclonal Antibody [JE54-56]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Kir2.1 aa 91-140 / 427.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 48 kDa

Positive Control

293 cell lysates.

Conjugation

unconjugated

Clone Number

JE54-56

RRID

AB_3072347

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is characterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features.

Background References

1. Despang A. et. al. Functional dissection of the Sox9-Kcnj2 locus identifies nonessential and instructive roles of TAD architecture. Nat Genet. 2019 Aug

2. Fukumura S. et. al. Functional analysis of a double-point mutation in the KCNJ2 gene identified in a family with Andersen-Tawil syndrome. J Neurol Sci. 2019 Dec

Subcellular Location

Membrane.

UNIPROT

SwissProt: P63252 Human

SwissProt: P35561 Mouse

SwissProt: Q64273 Rat

Synonyms

Cardiac inward rectifier potassium channel antibody

HHBIRK 1 antibody

HHBIRK1 antibody

HHIRK 1 antibody

HHIRK1 antibody

HIRK 1 antibody

hIRK1 antibody

Inward rectifier K antibody

Inward rectifier K(+) channel Kir2.1 antibody

Inward rectifier potassium channel 2 antibody

Expand

Images

  • Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (<a href="/products/HA721227" style="font-weight: bold;text-decoration: underline;">HA721227</a>) at 1/1,000 dilution.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 30s;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721227" style="font-weight: bold;text-decoration: underline;">HA721227</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (HA721227) at 1/1,000 dilution.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

    Exposure time: 30s;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721227) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation