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AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]

Usd: 385

Specification

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Catalog# HA721238

AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]

Application

  • IHC-P

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant full length protein corresponding to Human AFP.

Species Reactivity

Human

Validated Applications

IHC-P, WB

Molecular Weight

Predicted band size: 69 kDa

Positive Control

Human placenta tissue, human poorly differentiated hepatocellular carcinoma tissue, HepG2 cell lysates.

Conjugation

unconjugated

Clone Number

PD00-71

RRID

AB_3072358

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200

  • WB

  • 1:1,000

Target

Function

Alpha-fetoprotein (AFP) is the most abundant plasma protein found in the human fetus. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. Alpha-Fetoprotein (AFP) is synthesized by the cells of the embryonic yolk sac, fetal liver and fetal intestinal tract. This secretory protein is synthesized primarily in the fetal liver whereas expression is repressed in adult liver. Anti-AFP has been immunohistochemically demonstrated in hepatocellular carcinoma (HCC) and shows no immunoreactivity in normal liver. AFP levels decrease soon after birth. In abnormal tissues, expression of AFP has been demonstrated in hepatocellular carcinoma, hepatoid adenocarcinoma, germ cell tumors and particularly yolk sac tumor. The anti-AFP antibody may be useful for the identification of neoplastic liver diseases, yolk sac tumors and mixed germ cell tumors.

Background References

1. Mizejewski GJ. Alpha-fetoprotein structure and function: relevance to isoforms, epitopes, and conformational variants. Exp Biol Med. 2001; 226: 377-408.

2. Lazarevich NL. Molecular mechanisms of alpha-fetoprotein gene expression. Biochemistry (Mosc). 2000; 65:117-33.

Subcellular Location

Secreted

UNIPROT

SwissProt: P02771 Human

Synonyms

Afp antibody

AFPD antibody

Alpha fetoglobulin antibody

Alpha fetoprotein antibody

Alpha fetoprotein precursor antibody

Alpha-1-fetoprotein antibody

Alpha-fetoglobulin antibody

Alpha-fetoprotein antibody

alpha-fetoprotein, Hereditary persistence of, included antibody

FETA antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-AFP antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human poorly differentiated hepatocellular carcinoma tissue with Rabbit anti-AFP antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human poorly differentiated hepatocellular carcinoma tissue with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human well-differentiated hepatocellular carcinoma tissue (negative) with Rabbit anti-AFP antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human well-differentiated hepatocellular carcinoma tissue (negative) with Rabbit anti-AFP antibody (HA721238) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721238) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of AFP on HepG2 cell lysates with Rabbit anti-AFP antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 69 kDa<br />Observed band size: 69 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721238" style="font-weight: bold;text-decoration: underline;">HA721238</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AFP on HepG2 cell lysates with Rabbit anti-AFP antibody (HA721238) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721238) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"