Human TNF-beta Recombinant Rabbit Monoclonal Antibody [PS01-19] - BSA and Azide free (Detector)
Overview
Product Name
Human TNF-beta Recombinant Rabbit Monoclonal Antibody [PS01-19] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant full length protein human TNF-beta.
Species Reactivity
Human
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 22 kDa
Positive Control
Recombinant TNF-β protein.
Conjugation
unconjugated
Clone Number
PS01-19
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration.
Target
Function
Tumor necrosis factor-beta (TNF-β) is a protein that in humans is encoded by the LTA gene. Belonging to the hematopoietic cell line, LT-α exhibits anti-proliferative activity and causes the cellular destruction of tumor cell lines. As a cytotoxic protein, LT-α performs a variety of important roles in immune regulation depending on the form that it is secreted as. Unlike other members of the TNF superfamily, LT-α is only found as a soluble homotrimer, when found at the cell surface it is found only as a heterotrimer with LTβ. LT-α has a significant impact on the maintenance of the immune system including the development of secondary lymphoid organs. Absence of LT-α leads to the disruption of gastrointestinal development, prevents Peyer's patch development, and results in a disorganized spleen. As a signaling molecule, LT-α is involved in the regulation of cell survival, proliferation, differentiation, and apoptosis. LT-α plays an important role in innate immune regulation and its presence has been shown to prevent tumor growth and destroy cancerous cell lines. In contrast, unregulated expression of LT-α can result in a constantly active signaling pathway, thus leading to uncontrolled cellular growth and creation of tumors. Hence depending on the context, LT-α may function to prevent growth of cancer cells or facilitate the development of tumors. Furthermore, LT-α effects depend on the type of organ it acts upon, type of cancer cells, cellular environment, gender, and time of effect during an immune response.
Background References
1. Fernandes MT, Dejardin E, dos Santos NR (April 2016). "Context-dependent roles for lymphotoxin-β receptor signaling in cancer development". Biochimica et Biophysica Acta (BBA) - Reviews on Cancer. 1865 (2): 204–19.
2. Bauer J, Namineni S, Reisinger F, Zöller J, Yuan D, Heikenwälder M (2012-01-01). "Lymphotoxin, NF-ĸB, and cancer: the dark side of cytokines". Digestive Diseases. 30 (5): 453–68.
Subcellular Location
Membrane, Secreted
UNIPROT
Synonyms
LTalpha antibody
DAMA-25N12.13-004 antibody
DIF antibody
LT alpha antibody
LT antibody
LT-alpha antibody
Lta antibody
Lymphotoxin alpha (TNF superfamily, member 1) antibody
Lymphotoxin alpha antibody
Lymphotoxin-alpha antibody
ExpandImages
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![Standard curve of TNF-β matched pair antibodies:<br /><br />Sandwich ELISA analysis of TNF-β matched pair antibodies<br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody <a href="/products/HA721242" style="font-weight: bold;text-decoration: underline;">HA721242</a> [PS01-05] diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant TNF-β protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody <a href="/products/HA721243" style="font-weight: bold;text-decoration: underline;">HA721243</a> [PS01-19] (Biotin, 500 ng/ml) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.<br /><br />Capture/detect antibodies, recombinant TNF-β protein, Streptavidin-HRP are diluted in 1% BSA/PBST.](https://storage.huabio.cn/huabio/productImg/HA721243_1.jpg?v=20260429161635)
Standard curve of TNF-β matched pair antibodies:
Sandwich ELISA analysis of TNF-β matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721242 [PS01-05] diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant TNF-β protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721243 [PS01-19] (Biotin, 500 ng/ml) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture/detect antibodies, recombinant TNF-β protein, Streptavidin-HRP are diluted in 1% BSA/PBST. -
Western blot analysis of Human TNF-beta on recombinant Human LTA protein (HA210742) with Rabbit anti-Human TNF-beta antibody (HA721243) at 1/2,000 dilution.
Lysates/proteins at 10 ng/Lane.
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721243) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"