acetyl-Histone H4 (K8) Recombinant Rabbit Monoclonal Antibody [PS01-25] (HA721249)
Catalog# HA721249
Rabbit Monoclonal to acetyl-Histone H4 (K8)
Tested for
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WB
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IF-Cell
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IHC-P
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ChIP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Histone H4 (acetyl K8) on different lysates with Rabbit anti-Histone H4 (acetyl K8) antibody (HA721249) at 1/500 dilution.
Lane 1: Hela cell lysate, 10 µg/Lane
Lane 2: NIH/3T3 cell lysate, 10 µg/Lane
Lane 3: PC-12 cell lysate, 10 µg/Lane
Predicted band size: 11 kDa
Observed band size: 14/16 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721249) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate, 10 µg/Lane
Lane 2: NIH/3T3 cell lysate, 10 µg/Lane
Lane 3: PC-12 cell lysate, 10 µg/Lane
Predicted band size: 11 kDa
Observed band size: 14/16 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721249) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.