Human IL-1 beta Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Detector) [PS00-82] (HA721279)
Catalog# HA721279
Rabbit Monoclonal to Human IL-1 beta
Tested for
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ELISA(Det)
Reacts with
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Human
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Dog
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
The binding activity of IL-1 beta (HA721279) with recombinant IL-1 beta protein.
Immobilized recombinant IL-1 beta protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721279) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Immobilized recombinant IL-1 beta protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721279) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.