MEF2C Recombinant Rabbit Monoclonal Antibody [JE35-45]
Catalog# HA721298
MEF2C Recombinant Rabbit Monoclonal Antibody [JE35-45]
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WB
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IF-Cell
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IHC-P
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IP
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FC
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Human
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Rat
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unconjugated
Overview
Product Name
MEF2C Recombinant Rabbit Monoclonal Antibody [JE35-45]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human MEF2C aa 400-473.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell, IHC-P, IP, FC
Molecular Weight
Predicted band size: 51 kDa
Positive Control
THP-1 cell lysate, Rat brain tissue lysate, THP-1, human brain tissue, rat brain tissue, rat heart tissue.
Conjugation
unconjugated
Clone Number
JE35-45
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
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IP
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1-2μg/sample
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FC
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1:1,000
Target
Function
Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architectur. Isoforms that lack the repressor domain are more active than isoform 1. Expressed in brain and skeletal muscle. Expression is highest during the early stages of postnatal development, at later stages levels greatly decrease.
Background References
1. Zhu B., Ramachandran B., Gulick T. Alternative pre-mRNA splicing governs expression of a conserved acidic transactivation domain in myocyte enhancer factor 2 factors of striated muscle and brain. J. Biol. Chem. 280:28749-28760 (2005)
2. Ma K., Chan J.K., Zhu G., Wu Z. Myocyte enhancer factor 2 acetylation by p300 enhances its DNA binding activity, transcriptional activity, and myogenic differentiation. Mol. Cell. Biol. 25:3575-3582 (2005).
Subcellular Location
Cytoplasm, Nucleus
Synonyms
C5DELq14.3 antibody
DEL5q14.3 antibody
MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
MADS box transcription enhancer factor 2, polypeptide C antibody
MEF2C antibody
MEF2C_HUMAN antibody
Myocyte enhancer factor 2C antibody
Myocyte specific enhancer factor 2C antibody
Myocyte-specific enhancer factor 2C antibody
OTTHUMP00000222409 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of MEF2C on different lysates with Rabbit anti-MEF2C antibody (HA721298) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51/48 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling MEF2C with Rabbit anti-MEF2C antibody (HA721298) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MEF2C antibody (HA721298) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MEF2C antibody (HA721298) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721298) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MEF2C antibody (HA721298) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721298) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-MEF2C antibody (HA721298) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721298) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
MEF2C was immunoprecipitated from 0.2 mg THP-1 cell lysate with HA721298 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721298 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate (input)
Lane 2: HA721298 IP in THP-1 cell lysate
Lane 3: Rabbit IgG instead of HA721298 in THP-1 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801 -
☑ Relative expression (RE)
Flow cytometric analysis of Jurkat (left, negative) and THP-1 (right, positive) cells labeling MEF2C.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721298, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"