ICOSLG / B7-H2 / CD275 Recombinant Rabbit Monoclonal Antibody [JE52-85]
Catalog# HA721299
ICOSLG / B7-H2 / CD275 Recombinant Rabbit Monoclonal Antibody [JE52-85]
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WB
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Human
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unconjugated
Overview
Product Name
ICOSLG / B7-H2 / CD275 Recombinant Rabbit Monoclonal Antibody [JE52-85]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human ICOSLG aa 250-302.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
JAR cell lysate, HUVEC cell lysate, HUVEC treated with 20ng/mL TNF-alpha for 24 hours cell lysate, Daudi cell lysate, Raji cell lysate.
Conjugation
unconjugated
Clone Number
JE52-85
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
ICOS ligand (ICOSLG, ICOSL or GL50) is a protein that in humans is encoded by the ICOSLG gene located at chromosome 21. ICOSLG has also been designated as CD275 (cluster of differentiation 275). ICOSLG is glycosylated transmembrane structure, which is classified as a member of the B7 family due to the significant homology with B7 family members. The B7/CD28 superfamily provides both positive and negative co-signals to immunocytes in immune responses. The interaction of ICOSLG with ICOS, the specific receptor for ICOSLG, is critically involved in the activation, proliferation, differentiation and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses. ICOSLG delivers a potent co-stimulatory signal to T cells when engaged by ICOS, resulting in T cell activation and proliferation. The existence of ICOS/ICOSLG signal in vivo is closely associated with many mouse autoimmune disease models. Conversely, the absence of ICOS/ICOSLG signal may be a good way to relieve autoimmune disease. In view of its critical function in regulating immunohomeostasis, ICOS signaling has aroused great attention in immunodiagnosis and therapy. The ICOS/ICOSLG axis has been shown to promote either antitumor T cell responses (when activated in Th1 and other Teff) or protumor responses when triggered in Tregs. Therefore, both agonistic and antagonistic monoclonal antibodies (mAbs) targeting this pathway are being investigated for cancer immunotherapy.
Background References
1. Shen S, Wang F, Chen L, Wang T, Hu Y, Zhang X (August 2011). "Immunoreactivity of two novel monoclonal antibodies against human inducible co-stimulator ligand". Hybridoma. 30 (4): 361–368.
2. Amatore F, Gorvel L, Olive D (April 2018). "Inducible Co-Stimulator (ICOS) as a potential therapeutic target for anti-cancer therapy". Expert Opinion on Therapeutic Targets. 22 (4): 343–351.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
B7 H2 antibody
B7 homolog 2 antibody
B7 homologue 2 antibody
B7 like protein Gl50 antibody
B7 related protein 1 antibody
B7-H2 antibody
B7-like protein Gl50 antibody
B7-related protein 1 antibody
B7H2 antibody
B7RP 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of ICOSLG / B7-H2 / CD275 on different lysates with Rabbit anti-ICOSLG / B7-H2 / CD275 antibody (HA721299) at 1/1,000 dilution.
Lane 1: JAR cell lysate
Lane 2: Jurkat cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 60 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721299) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of ICOSLG / B7-H2 / CD275 on different lysates with Rabbit anti-ICOSLG / B7-H2 / CD275 antibody (HA721299) at 1/500 dilution.
Lane 1: HUVEC cell lysate
Lane 2: HUVEC treated with 20ng/mL TNF-alpha for 24 hours cell lysate
Lane 3: Daudi cell lysate
Lane 4: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 65 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721299) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"