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Cdc6 Recombinant Rabbit Monoclonal Antibody [JE59-52]

Usd: 385

Specification

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Catalog# HA721304

Cdc6 Recombinant Rabbit Monoclonal Antibody [JE59-52]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Cdc6 Recombinant Rabbit Monoclonal Antibody [JE59-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser54 of Human Cdc6 aa 41-90 / 560.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 63 kDa

Positive Control

Hela cell lysate, HepG2 cell lysate, human striated muscle tissue, human testis muscle tissue.

Conjugation

unconjugated

Clone Number

JE59-52

RRID

AB_3072421

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:200

Target

Function

Cdc6, or cell division cycle 6, is a protein in eukaryotic cells. CDC6 is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). CDC6 assembles after ORC in an ATP dependent manner and is required for loading MCM proteins onto the DNA. It is thought that the CDC6-Cdt1 complex uses ATP hydrolysis to thread DNA through the central hole of the MCM doughnut. Mutations in the binding motif of CDC6 strongly suggest that ATP binding and hydrolysis is essential for its function. The minimal requirement for DNA binding has been mapped within its 47-amino acid sequence. Furthermore, Cdc6 indirectly inhibits activation of the p34cdc2/CDC28 M phase kinase, thus nuclear division is suppressed.

Background References

1. Fan X et al. Role of Cdc6 in re-replication in cells expressing human papillomavirus E7 oncogene. Carcinogenesis 37:799-809 (2016).

2. Huang J et al. miR-34a Modulates Angiotensin II-Induced Myocardial Hypertrophy by Direct Inhibition of ATG9A Expression and Autophagic Activity. PLoS One 9:e94382 (2014).

Sequence Similarity

Belongs to the CDC6/cdc18 family.

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q99741 Human

Synonyms

Cdc 18L antibody

Cdc 6 antibody

CDC18 (cell division cycle 18, S.pombe, homolog) like antibody

CDC18 (S.pombe) antibody

Cdc18 related protein antibody

CDC18(S.pombe) antibody

Cdc18-related protein antibody

Cdc18L antibody

cdc6 antibody

CDC6 cell division cycle 6 homolog antibody

Expand

Images

  • Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (<a href="/products/HA721304" style="font-weight: bold;text-decoration: underline;">HA721304</a>) at 1/500 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: HepG2 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721304" style="font-weight: bold;text-decoration: underline;">HA721304</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (HA721304) at 1/500 dilution.

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721304) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Cdc6 antibody (<a href="/products/HA721304" style="font-weight: bold;text-decoration: underline;">HA721304</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721304" style="font-weight: bold;text-decoration: underline;">HA721304</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Cdc6 antibody (HA721304) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721304) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis muscle tissue with Rabbit anti-Cdc6 antibody (<a href="/products/HA721304" style="font-weight: bold;text-decoration: underline;">HA721304</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721304" style="font-weight: bold;text-decoration: underline;">HA721304</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis muscle tissue with Rabbit anti-Cdc6 antibody (HA721304) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721304) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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