NF-kB p65 Recombinant Rabbit Monoclonal Antibody [PSH0-27] (HA721307)
Catalog# HA721307
Rabbit Monoclonal to NF-kB p65
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
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Monkey
Lane 1: HeLa cell lysate (control) (35 µg/Lane)
Lane 2: HeLa-RELA-KO (1) (35 µg/Lane)
Lane 3: HeLa-RELA-KO (2) (35 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 65 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721307) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NF-kB p65 Recombinant Rabbit Monoclonal Antibody [PSH0-27] (HA721307)
Immunogen
Synthetic peptide within human rela aa 502-551 / 551.
Host
Rabbit
Positive Control
HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, A431 cell lysate, Raji cell lysate, HL-60 cell lysate, Daudi cell lysate, COS-1 cell lysate, Mouse lung tissue lysate, human breast cancer tissue, human breast tissue, HeLa, NIH/3T3 cells treated with 50μg/mL TNF-α for 20 minutes, PC-12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH0-27
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:100-1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 65 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NF-kB p65
SYNONYMS
Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody;MGC131774 antibody;NF kappa B p65delta3 antibody;nfkappabp65 antibody;NFkB p65 antibody;NFKB3 antibody;Nuclear factor kappaB antibody;Nuclear Factor NF Kappa B p65 Subunit antibody;Nuclear factor NF-kappa-B p65 subunit antibody;Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody;OTTHUMP00000233473 antibody;OTTHUMP00000233474 antibody;OTTHUMP00000233475 antibody;OTTHUMP00000233476 antibody;OTTHUMP00000233900 antibody;p65 antibody;p65 NF kappaB antibody;p65 NFkB antibody;relA antibody;TF65_HUMAN antibody;Transcription factor NFKB3 antibody;Transcription factor p65 antibody;v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody;V rel avian reticuloendotheliosis viral oncogene homolog A antibody;v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody;V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
Transcription factor p65 also known as nuclear factor NF-kappa-B p65 subunit is a protein that in humans is encoded by the RELA gene. RELA, also known as p65, is a REL-associated protein involved in NF-κB heterodimer formation, nuclear translocation and activation. NF-κB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc. Phosphorylation and acetylation of RELA are crucial post-translational modifications required for NF-κB activation. RELA has also been shown to modulate immune responses, and activation of RELA is positively associated with multiple types of cancer.
CITATIONS
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MicroRNA-579-3p Exerts Neuroprotective Effects Against Ischemic Stroke via Anti-Inflammation and Anti-Apoptosis
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WB
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Rat
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Citation
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Intra-bone marrow injection of magnesium isoglyrrhizinate inhibits inflammation and delays osteoarthritis progression through the NF-κB pathway
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WB
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Rabbit
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Citation
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NecroX-5 alleviate lipopolysaccharide-induced acute respiratory distress syndrome by inhibiting TXNIP/NLRP3 and NF-κB
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WB
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Mouse
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Citation
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Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65
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WB
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Human
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Citation
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Chen Jialei,et al
A Rapid Self-Assembling Peptide Hydrogel for Delivery of TFF3 to Promote Gastric Mucosal Injury Repair
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WB
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Human
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Citation
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Wu Lianchi,et al
Hericium erinaceus polysaccharides ameliorate nonalcoholic fatty liver disease via gut microbiota and tryptophan metabolism regulation in an aged laying hen model
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Hen
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Citation
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Zhou Yiqing,et al
Buddleoside-rich Chrysanthemum indicum L. extract modulates macrophage-mediated inflammation to prevent metabolic syndrome induced by unhealthy diet
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WB
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Rat
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Citation
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