ZEB1 Recombinant Rabbit Monoclonal Antibody [JE57-64]
Catalog# HA721318
ZEB1 Recombinant Rabbit Monoclonal Antibody [JE57-64]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
ZEB1 Recombinant Rabbit Monoclonal Antibody [JE57-64]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ZEB1 aa 1-180/1124.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 124 kDa
Positive Control
Hela cell lysate, HUVEC cell lysate, human lung carcinoma tissue.
Conjugation
unconjugated
Clone Number
JE57-64
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
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IHC-P
-
1:200
Target
Function
Zinc finger E-box-binding homeobox 1 is a protein that in humans is encoded by the ZEB1 gene. ZEB1 (previously known as TCF8) encodes a zinc finger and homeodomain transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site. ZEB1 and its mammalian paralog ZEB2 belongs to the Zeb family within the ZF (zinc finger) class of homeodomain transcription factors. ZEB1 protein has 7 zinc fingers and 1 homeodomain. The structure of the homeodomain is shown on the right. Mutations of the gene are linked to posterior polymorphous corneal dystrophy 3. ZEB1 downregulates E-cadherin and induces epithelial to mesenchymal transition in breast and other carcinomas A recent study suggested its contributing role in lung cancer invasiveness and metastasis development. Overexpression of ZEB1 has been identified as a potential risk factor for recurrence and poor prognosis in several types of cancers.
Background References
1. Zhang Y et al. Expression and Function of ZEB1 in the Cornea. Cells. 2021 Apr
2. Zhou Y et al. ZEB1 enhances Warburg effect to facilitate tumorigenesis and metastasis of HCC by transcriptionally activating PFKM. Theranostics. 2021 Apr
Subcellular Location
Nucleus.
UNIPROT
Synonyms
AREB 6 antibody
AREB6 antibody
BZP antibody
Delta crystallin enhancer binding factor 1 antibody
DELTA EF1 antibody
FECD6 antibody
MGC133261 antibody
Negative regulator of IL 2 antibody
Negative regulator of IL2 antibody
NIL 2 A antibody
ExpandImages
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Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (HA721318) at 1/500 dilution.
Lane 1: HUVEC cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721318) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-ZEB1 antibody (HA721318) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721318) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"