Skip to content

SPOP Recombinant Rabbit Monoclonal Antibody [JE36-04]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721319

SPOP Recombinant Rabbit Monoclonal Antibody [JE36-04]

Application

  • WB

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SPOP Recombinant Rabbit Monoclonal Antibody [JE36-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SPOP aa 291-340 / 374.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

K-562 (Human chronic myelogenous leukemia cell) cell lysate, LoVo (Human colon cancer cell) cell lysate.

Conjugation

unconjugated

Clone Number

JE36-04

RRID

AB_3072436

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Speckle-type POZ protein is a protein that in humans is encoded by the SPOP gene. This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. Mutations in SPOP lead to a type of prostate tumor thought to be involved in about 15% of all prostate cancers.

Background References

1. Song Y et al. The emerging role of SPOP protein in tumorigenesis and cancer therapy. Mol Cancer. 2020 Jan

2. Zhang J et al. SPOP mutation induces DNA methylation via stabilizing GLP/G9a. Nat Commun. 2021 Sep

Subcellular Location

Nucleus, Nucleus speckle.

UNIPROT

SwissProt: O43791 Human

SwissProt: Q6ZWS8 Mouse

Synonyms

BTBD32 antibody

HIB homolog 1 antibody

Roadkill homolog 1 antibody

Speckle type POZ protein antibody

Speckle-type POZ protein antibody

SPOP antibody

SPOP_HUMAN antibody

TEF 2 antibody

TEF2 antibody

Images

  • Western blot analysis of SPOP on different lysates with Rabbit anti-SPOP antibody (<a href="/products/HA721319" style="font-weight: bold;text-decoration: underline;">HA721319</a>) at 1/2,000 dilution.<br /><br />Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate<br />Lane 2: LoVo (Human colon cancer cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 30 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721319" style="font-weight: bold;text-decoration: underline;">HA721319</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa

    Western blot analysis of SPOP on different lysates with Rabbit anti-SPOP antibody (HA721319) at 1/2,000 dilution.

    Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
    Lane 2: LoVo (Human colon cancer cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721319, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation