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EAAT2 Recombinant Rabbit Monoclonal Antibody [PS01-62]

Usd: 385

Specification

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Catalog# HA721323

EAAT2 Recombinant Rabbit Monoclonal Antibody [PS01-62]

Application

  • WB

  • IHC-P

  • IHC-Fr

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Human

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

EAAT2 Recombinant Rabbit Monoclonal Antibody [PS01-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human EAAT2

Species Reactivity

Mouse, Rat (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr

Molecular Weight

Predicted band size: 62 kDa

Positive Control

Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

PS01-62

RRID

AB_3072440

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000

  • IHC-Fr

  • 1:200-1:500

Target

Function

Excitatory amino acid transporter 2 (EAAT2) also known as solute carrier family 1 member 2 (SLC1A2) and glutamate transporter 1 (GLT-1) is a protein that in humans is encoded by the SLC1A2 gene. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. SLC1A2 / EAAT2 is a member of a family of the solute carrier family of proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. EAAT2 is responsible for over 90% of glutamate reuptake within the brain.

Background References

1. Blacker CJ et al. EAAT2 as a Research Target in Bipolar Disorder and Unipolar Depression: A Systematic Review. Mol Neuropsychiatry. 2020 Apr

2. Green JL et al. Role of glutamate excitotoxicity and glutamate transporter EAAT2 in epilepsy: Opportunities for novel therapeutics development. Biochem Pharmacol. 2021 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P43004 Human

SwissProt: P43006 Mouse

SwissProt: P31596 Rat

Synonyms

EAA2_HUMAN antibody

EAAT2 antibody

Excitatory amino acid transporter 2 antibody

Excitotoxic amino acid transporter 2 antibody

Glial high affinity glutamate transporter antibody

GLT 1 antibody

GLT1 antibody

Glutamate aspartate transporter II antibody

Glutamate transporter 1 antibody

Glutamate/aspartate transporter II antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:200

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of EAAT2 on different lysates with Rabbit anti-EAAT2 antibody (<a href="/products/HA721323" style="font-weight: bold;text-decoration: underline;">HA721323</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (no heat)<br />Lane 2: Mouse cerebellum tissue lysate (no heat)<br />Lane 3: Mouse lung tissue lysate (negative) (no heat)<br />Lane 4: Rat brain tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721323" style="font-weight: bold;text-decoration: underline;">HA721323</a>) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of EAAT2 on different lysates with Rabbit anti-EAAT2 antibody (HA721323) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (no heat)
    Lane 2: Mouse cerebellum tissue lysate (no heat)
    Lane 3: Mouse lung tissue lysate (negative) (no heat)
    Lane 4: Rat brain tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    Exposure time: 4 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721323) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EAAT2 antibody (<a href="/products/HA721323" style="font-weight: bold;text-decoration: underline;">HA721323</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721323" style="font-weight: bold;text-decoration: underline;">HA721323</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EAAT2 antibody (HA721323) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721323) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-EAAT2 antibody (<a href="/products/HA721323" style="font-weight: bold;text-decoration: underline;">HA721323</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721323" style="font-weight: bold;text-decoration: underline;">HA721323</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-EAAT2 antibody (HA721323) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721323) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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