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CRALBP Recombinant Rabbit Monoclonal Antibody [JE35-09]

Usd: 385

Specification

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Catalog# HA721330

CRALBP Recombinant Rabbit Monoclonal Antibody [JE35-09]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CRALBP Recombinant Rabbit Monoclonal Antibody [JE35-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CRALBP aa 26-75 / 317.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 36.5 kDa

Positive Control

Rat eyeball tissue lysate, mouse eyeball tissue lysate, B16F1.

Conjugation

unconjugated

Clone Number

JE35-09

RRID

AB_3072447

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50

  • FC

  • 1ug/mL

Target

Function

Retinaldehyde-binding protein 1 (RLBP1) also known as cellular retinaldehyde-binding protein (CRALBP) is a 36-kD water-soluble protein that in humans is encoded by the RLBP1 gene. Cellular retinol binding protein (CRBP) was first discovered in 1973 from lung tissues by Bashor et al. There have been three cellular retinol binding protein categories discovered; Cellular retinol-binding protein, cellular retinoic acid-binding protein and cellular retinaldehyde-binding protein(CRALBP). CRALBP was first discovered in 1977, after it was purified from retina and retinal pigment epithelial cells. The cellular retinaldehyde-binding protein transports 11-cis-retinal (also known as 11-cis-retinaldehyde) as its physiological ligands. It plays a critical role as an 11-cis-retinal acceptor which facilitates the enzymatic isomerization of all 11-trans-retinal to 11-cis-retinal, in the isomerization of the rod and cones of the visual cycle.

Background References

1. Bocquet B et al. Retinitis Punctata Albescens and RLBP1-Allied Phenotypes: Phenotype-Genotype Correlation and Natural History in the Aim of Gene Therapy. Ophthalmol Sci. 2021 Aug

2. Lima de Carvalho JR Jr et al. Effects of deficiency in the RLBP1-encoded visual cycle protein CRALBP on visual dysfunction in humans and mice. J Biol Chem. 2020 May

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9Z275 Mouse

Entrez Gene: 293049 Rat

Synonyms

Cellular retinaldehyde binding protein 1 antibody

Cellular retinaldehyde binding protein antibody

Cellular retinaldehyde-binding protein antibody

MGC3663 antibody

Retinaldehyde binding protein 1 antibody

Retinaldehyde-binding protein 1 antibody

RLBP 1 antibody

RLBP1 antibody

RLBP1_HUMAN antibody

Images

  • Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (<a href="/products/HA721330" style="font-weight: bold;text-decoration: underline;">HA721330</a>) at 1/500 dilution.<br /><br />Lane 1: Rat eyeball tissue lysate<br />Lane 2: Mouse eyeball tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 36.5 kDa<br />Observed band size: 36.5 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721330" style="font-weight: bold;text-decoration: underline;">HA721330</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (HA721330) at 1/500 dilution.

    Lane 1: Rat eyeball tissue lysate
    Lane 2: Mouse eyeball tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 36.5 kDa
    Observed band size: 36.5 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721330) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of B16F1 cells labeling CRALBP with Rabbit anti-CRALBP antibody (<a href="/products/HA721330" style="font-weight: bold;text-decoration: underline;">HA721330</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRALBP antibody (<a href="/products/HA721330" style="font-weight: bold;text-decoration: underline;">HA721330</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of B16F1 cells labeling CRALBP with Rabbit anti-CRALBP antibody (HA721330) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRALBP antibody (HA721330) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of B16F1 cells labeling CRALBP.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721330" style="font-weight: bold;text-decoration: underline;">HA721330</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of B16F1 cells labeling CRALBP.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721330, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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