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LYPD3 Recombinant Rabbit Monoclonal Antibody [PS00-59]

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Specification

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Catalog# HA721332

LYPD3 Recombinant Rabbit Monoclonal Antibody [PS00-59]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

LYPD3 Recombinant Rabbit Monoclonal Antibody [PS00-59]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human LYPD3 31-326.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 36 kDa

Positive Control

SK-Br-3 cell lysate, MCF-7 cell lysate, A431 cell lysate, human esophagus tissue, human tonsil tissue.

Conjugation

unconjugated

Clone Number

PS00-59

RRID

AB_3072449

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:2,000

Target

Function

C4.4A (Ly6/PLAUR domain-containing protein 3, LYPD3) is a tumorigenic and high-glycosylated cell surface protein that has been proven to be linked with the carcinogenic effects in different solid tumors. The elevated expression of LYPD3 is not only demonstrated to be associated with lung adenocarcinoma carcinogenesis and poor prognosis but also there is evidence that LYPD3 can lead to the initiation and development of cancers and the chemoresistance of metastatic cancers by impacting the proliferation and apoptosis of the tumor, which are involved in many important regulatory mechanisms of cancers. LYPD3 can be the ideal target for the therapy method and early detection of AML.

Background References

1. Hu T et al. LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia. Front Genet. 2022 Mar

2. Hu P et al. Elevated Expression of LYPD3 Is Associated with Lung Adenocarcinoma Carcinogenesis and Poor Prognosis. DNA Cell Biol. 2020 Apr

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: O95274 Human

Synonyms

2310061G07Rik antibody

C4.4A antibody

GPI-anchored metastasis-associated protein C4.4A homolog antibody

GPI-anchored metastasis-associated protein homolog antibody

Ly6/PLAUR domain-containing protein 3 antibody

Lypd3 antibody

LYPD3_HUMAN antibody

Matrigel-induced gene C4 protein antibody

MIG-C4 antibody

SHINC3 antibody

Images

  • Western blot analysis of LYPD3 on different lysates with Rabbit anti-LYPD3 antibody (<a href="/products/HA721332" style="font-weight: bold;text-decoration: underline;">HA721332</a>) at 1/1,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate<br />Lane 2: MCF-7 cell lysate<br />Lane 3: A431 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 1 minute 17 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721332" style="font-weight: bold;text-decoration: underline;">HA721332</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LYPD3 on different lysates with Rabbit anti-LYPD3 antibody (HA721332) at 1/1,000 dilution.

    Lane 1: SK-Br-3 cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: A431 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 90 kDa

    Exposure time: 1 minute 17 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721332) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-LYPD3 antibody (<a href="/products/HA721332" style="font-weight: bold;text-decoration: underline;">HA721332</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721332" style="font-weight: bold;text-decoration: underline;">HA721332</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-LYPD3 antibody (HA721332) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721332) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LYPD3 antibody (<a href="/products/HA721332" style="font-weight: bold;text-decoration: underline;">HA721332</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721332" style="font-weight: bold;text-decoration: underline;">HA721332</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LYPD3 antibody (HA721332) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721332) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation