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PRMT6 Recombinant Rabbit Monoclonal Antibody [JE36-57]

Usd: 385

Specification

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Catalog# HA721333

PRMT6 Recombinant Rabbit Monoclonal Antibody [JE36-57]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PRMT6 Recombinant Rabbit Monoclonal Antibody [JE36-57]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PRMT6 aa 191-240 / 375.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

Human kidney tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, rat kidney tissue lysate, rat testis tissue lysate, 293T cell lysate, HEK-293 cell lysate.

Conjugation

unconjugated

Clone Number

JE36-57

RRID

AB_3072450

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Protein arginine N-methyltransferase 6 is an enzyme that in humans is encoded by the PRMT6 gene. Protein arginine N-methyltransferases, such as PRMT6, catalyze the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine.

Background References

1. Chen Z et al. The Emerging Role of PRMT6 in Cancer. Front Oncol. 2022 Mar

2. Huang T et al. PRMT6 methylation of RCC1 regulates mitosis, tumorigenicity, and radiation response of glioblastoma stem cells. Mol Cell. 2021 Mar

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q96LA8 Human

SwissProt: Q6NZB1 Mouse

Entrez Gene: 295384 Rat

Synonyms

ANM6_HUMAN antibody

Chromobox protein homolog 7 antibody

FLJ10559 antibody

FLJ51477 antibody

Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibody

Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody

Histone-arginine N-methyltransferase PRMT6 antibody

HMT1 hnRNP methyltransferase like 6. antibody

HRMT1L6 antibody

OTTHUMP00000012633 antibody

Expand

Images

  • Western blot analysis of PRMT6 on different lysates with Rabbit anti-PRMT6 antibody (<a href="/products/HA721333" style="font-weight: bold;text-decoration: underline;">HA721333</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human kidney tissue lysate (20 µg/Lane)<br />Lane 2: Mouse kidney tissue lysate (20 µg/Lane)<br />Lane 3: Mouse testis tissue lysate (20 µg/Lane)<br />Lane 4: Rat kidney tissue lysate (20 µg/Lane)<br />Lane 5: Rat testis tissue lysate (20 µg/Lane)<br />Lane 6: 293T cell lysate (10 µg/Lane)<br />Lane 7: HEK-293 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa (Unknown band detected in mouse tissue)<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721333" style="font-weight: bold;text-decoration: underline;">HA721333</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PRMT6 on different lysates with Rabbit anti-PRMT6 antibody (HA721333) at 1/1,000 dilution.

    Lane 1: Human kidney tissue lysate (20 µg/Lane)
    Lane 2: Mouse kidney tissue lysate (20 µg/Lane)
    Lane 3: Mouse testis tissue lysate (20 µg/Lane)
    Lane 4: Rat kidney tissue lysate (20 µg/Lane)
    Lane 5: Rat testis tissue lysate (20 µg/Lane)
    Lane 6: 293T cell lysate (10 µg/Lane)
    Lane 7: HEK-293 cell lysate (10 µg/Lane)

    Predicted band size: 42 kDa
    Observed band size: 42 kDa (Unknown band detected in mouse tissue)

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721333) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation