RTN3 Recombinant Rabbit Monoclonal Antibody [PSH0-40]
Catalog# HA721342
RTN3 Recombinant Rabbit Monoclonal Antibody [PSH0-40]
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WB
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IF-Cell
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FC
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Human
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Rat
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unconjugated
Overview
Product Name
RTN3 Recombinant Rabbit Monoclonal Antibody [PSH0-40]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human RTN3 aa 1-500 / 1,032.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 113 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, U87-MG cell lysate, A549 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, L6 cell lysate, human cerebellum tissue lysate, PC-12, HeLa.
Conjugation
unconjugated
Clone Number
PSH0-40
RRID
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
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| Rat |
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|
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| Mouse |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50
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FC
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1:500-1:1,000
Target
Function
May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. Induces the formation of endoplasmic reticulum tubules. Acts also as an inflammation-resolving regulator by interacting with both TRIM25 and RIG-I/DDX58, subsequently impairing DDX58 'Lys-63'-linked polyubiquitination leading to IRF3 and NF-kappa-B inhibition. This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12.
Background References
1. Yang Z et al. RTN3 inhibits RIG-I-mediated antiviral responses by impairing TRIM25-mediated K63-linked polyubiquitination. Elife. 2021 Jul
2. Qian C et al. RTN3 - ASC interaction: The potential mechanism behind diabetes-induced cortical neuritic dystrophy. Neurosci Lett. 2022 Jan
Subcellular Location
Endoplasmic reticulum membrane, Golgi apparatus membrane.
Synonyms
ASYIP antibody
Neuroendocrine specific protein like 2 antibody
Neuroendocrine-specific protein-like 2 antibody
Neuroendocrine-specific protein-like II antibody
NSP like protein II antibody
NSP-like protein 2 antibody
NSP-like protein II antibody
NSPL2 antibody
NSPLII antibody
Reticulon 3 antibody
ExpandImages
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Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA721342) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (32 µg/Lane)
Lane 2: HEK-293 cell lysate (33 µg/Lane)
Lane 3: U87-MG cell lysate (30 µg/Lane)
Lane 4: A549 cell lysate (26 µg/Lane)
Lane 5: SH-SY5Y cell lysate (18 µg/Lane)
Lane 6: PC-12 cell lysate (23 µg/Lane)
Lane 7: L6 cell lysate (14 µg/Lane)
Lane 8: Human cerebellum tissue lysate (40 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721342) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA721342) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-RTN3 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721342) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling RTN3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721342, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"