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Cathepsin H Recombinant Rabbit Monoclonal Antibody [JE56-25]

Usd: 385

Specification

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Catalog# HA721352

Cathepsin H Recombinant Rabbit Monoclonal Antibody [JE56-25]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Cathepsin H Recombinant Rabbit Monoclonal Antibody [JE56-25]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Cathepsin H aa 180-230.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 37 kDa

Positive Control

PC-3M cell lysates, PANC-1.

Conjugation

unconjugated

Clone Number

JE56-25

RRID

AB_3072469

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50

Target

Function

Cathepsin H is a protein that in humans is encoded by the CTSH gene. The protein encoded by this gene is a cysteine cathepsin, a lysosomal cysteine protease important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Two transcript variants encoding different isoforms have been found for this gene.

Background References

1. Peng P et al. Favorable Prognostic Impact of Cathepsin H (CTSH) High Expression in Thyroid Carcinoma. Int J Gen Med. 2021 Sep

2. Ye J et al. Genetic and environmental factors regulate the type 1 diabetes gene CTSH via differential DNA methylation. J Biol Chem. 2021 Jan-Jun

Subcellular Location

Lysosome.

UNIPROT

SwissProt: P09668 Human

Synonyms

Pro-cathepsin H

Cathepsin H mini chain

Cathepsin H

EC:3.4.22.16

Cathepsin H heavy chain

Cathepsin H light chain

CTSH

CPSB

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cathepsin H on different lysates with Rabbit anti-Cathepsin H antibody (<a href="/products/HA721352" style="font-weight: bold;text-decoration: underline;">HA721352</a>) at 1/500 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si Cathepsin H cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721352" style="font-weight: bold;text-decoration: underline;">HA721352</a>) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cathepsin H on different lysates with Rabbit anti-Cathepsin H antibody (HA721352) at 1/500 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si Cathepsin H cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 42 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721352) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cathepsin H on PC-3M cell lysates with Rabbit anti-Cathepsin H antibody (<a href="/products/HA721352" style="font-weight: bold;text-decoration: underline;">HA721352</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 3 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721352" style="font-weight: bold;text-decoration: underline;">HA721352</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cathepsin H on PC-3M cell lysates with Rabbit anti-Cathepsin H antibody (HA721352) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 42 kDa

    Exposure time: 3 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721352) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of PANC-1 cells labeling Cathepsin H with Rabbit anti-Cathepsin H antibody (<a href="/products/HA721352" style="font-weight: bold;text-decoration: underline;">HA721352</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin H antibody (<a href="/products/HA721352" style="font-weight: bold;text-decoration: underline;">HA721352</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of PANC-1 cells labeling Cathepsin H with Rabbit anti-Cathepsin H antibody (HA721352) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin H antibody (HA721352) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"