P70 S6 Kinase beta Recombinant Rabbit Monoclonal Antibody [JE35-69] (HA721354)
Catalog# HA721354
Rabbit Monoclonal to P70 S6 Kinase beta
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WB
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IF-Cell
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FC
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IHC-P
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Human
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Mouse
Lane 1: HeLa cell lysate, 20 µg/Lane
Lane 2: Jurkat cell lysate, 20 µg/Lane
Lane 3: HEK-293 cell lysate, 20 µg/Lane
Lane 4: Mouse liver tissue lysate, 40 µg/Lane
Lane 5: Mouse kidney tissue lysate, 40 µg/Lane
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721354) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
P70 S6 Kinase beta Recombinant Rabbit Monoclonal Antibody [JE35-69] (HA721354)
Immunogen
Recombinant protein within human p70 s6 kinase beta aa 1-150 / 482.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, HeLa, MCF7, human colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE35-69
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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FC
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1:500-1:1,000
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
P70 S6 Kinase beta
SYNONYMS
70 kDa ribosomal protein S6 kinase 2 antibody;EC 2.7.11.1 antibody;KS6B2_HUMAN antibody;p70 beta antibody;p70 ribosomal S6 kinase beta antibody;p70 S6 kinase beta antibody;p70 S6K-beta antibody;p70 S6KB antibody;p70 S6Kbeta antibody;p70(S6K) beta antibody;p70-beta antibody;p70-S6K 2 antibody;P70S6K2 antibody;p70S6Kb antibody;Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody;Ribosomal protein S6 kinase B2 antibody;Ribosomal protein S6 kinase beta 2 antibody;Ribosomal protein S6 kinase beta-2 antibody;Rps6kb2 antibody;S6 kinase related kinase antibody;S6 kinase-related kinase antibody;S6K beta 2 antibody;S6K beta antibody;S6K-beta antibody;S6K-beta-2 antibody;S6K2 antibody;Serine/threonine protein kinase 14 beta antibody;Serine/threonine-protein kinase 14B antibody;SRK antibody;STK14B antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Ribosomal protein S6 kinase beta-2 is an enzyme that in humans is encoded by the RPS6KB2 gene. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.
CITATIONS
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Autophagy participates in germline cyst breakdown and follicular formation by modulating glycolysis switch via Akt signaling in newly-hatched chicken ovaries
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WB
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chicken
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Citation
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