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TR4 Recombinant Rabbit Monoclonal Antibody [JE45-03]

Usd: 385

Specification

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Catalog# HA721372

TR4 Recombinant Rabbit Monoclonal Antibody [JE45-03]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

TR4 Recombinant Rabbit Monoclonal Antibody [JE45-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TR4 aa 497-596 / 596.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 65 kDa

Positive Control

Hela cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, human pancreas tissue, mouse pancreas tissue.

Conjugation

unconjugated

Clone Number

JE45-03

RRID

AB_3072489

Product Features

Form

Liquid

Concentration

1mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity).

Background References

1. Lee Y.F et al. Differential regulation of direct repeat 3 vitamin D3 and direct repeat 4 thyroid hormone signaling pathways by the human TR4 orphan receptor. J. Biol. Chem. 274:16198-16205 (1999)

2. Tanabe O et al. The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription. Genes Dev. 21:2832-2844 (2007)

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P49116 Human

SwissProt: P49117 Mouse

SwissProt: P55094 Rat

Synonyms

hTAK1 antibody

Nr2c2 antibody

NR2C2_HUMAN antibody

Nuclear hormone receptor TR4 antibody

Nuclear receptor subfamily 2 group C member 2 antibody

Orphan nuclear receptor TAK1 antibody

Orphan nuclear receptor TR4 antibody

TAK1 antibody

Testicular nuclear receptor 4 antibody

Testicular receptor 4 antibody

Expand

Images

  • Western blot analysis of TR4 on different lysates with Rabbit anti-TR4 antibody (<a href="/products/HA721372" style="font-weight: bold;text-decoration: underline;">HA721372</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: SH-SY5Y cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: PC-3M cell lysate<br />Lane 5: NIH/3T3 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721372" style="font-weight: bold;text-decoration: underline;">HA721372</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TR4 on different lysates with Rabbit anti-TR4 antibody (HA721372) at 1/1,000 dilution.

    Lane 1: Hela cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: PC-3M cell lysate
    Lane 5: NIH/3T3 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721372) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TR4 antibody (<a href="/products/HA721372" style="font-weight: bold;text-decoration: underline;">HA721372</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721372" style="font-weight: bold;text-decoration: underline;">HA721372</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TR4 antibody (HA721372) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721372) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-TR4 antibody (<a href="/products/HA721372" style="font-weight: bold;text-decoration: underline;">HA721372</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721372" style="font-weight: bold;text-decoration: underline;">HA721372</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-TR4 antibody (HA721372) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721372) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"