NR5A2 Recombinant Rabbit Monoclonal Antibody [JE30-50]
Catalog# HA721379
NR5A2 Recombinant Rabbit Monoclonal Antibody [JE30-50]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NR5A2 Recombinant Rabbit Monoclonal Antibody [JE30-50]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human NR5A2 aa 442-541 / 541.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 61 kDa
Positive Control
MCF7 cell lysate, 293T cell lysate, HepG2 cell lysate, A549 cell lysate, PANC-1 cell lysate, SW1990 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse pancreas tissue lysate.
Conjugation
unconjugated
Clone Number
JE30-50
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The liver receptor homolog-1 (LRH-1) also known as totipotency pioneer factor NR5A2 (nuclear receptor subfamily 5, group A, member 2) is a protein that in humans is encoded by the NR5A2 gene. LRH-1 is a member of the nuclear receptor family of intracellular transcription factors. LRH-1 plays a critical role in the regulation of development, cholesterol transport, bile acid homeostasis and steroidogenesis. LRH-1 is important for maintaining pluripotence of stem cells during embryonic development. Liver receptor homolog-1 has been shown to interact with the small heterodimer partner.
Background References
1. Gassler J et al. Zygotic genome activation by the totipotency pioneer factor Nr5a2. Science. 2022 Dec
2. Hughes CHK et al. NR5A2 and potential regulatory miRNAs in the bovine CL during early pregnancy. Reproduction. 2021 Feb
Subcellular Location
Nucleus.
Synonyms
Alpha-1-fetoprotein transcription factor antibody
B1-binding factor antibody
B1F antibody
B1F2 antibody
CPF antibody
CYP7A promoter-binding factor antibody
FTF antibody
FTZ F1 antibody
FTZ F1beta antibody
FTZ-F1 antibody
ExpandImages
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Western blot analysis of NR5A2 on different lysates with Rabbit anti-NR5A2 antibody (HA721379) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: 293T cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: A549 cell lysate (15 µg/Lane)
Lane 5: PANC-1 cell lysate (15 µg/Lane)
Lane 6: SW1990 cell lysate (15 µg/Lane)
Lane 7: Mouse liver tissue lysate (30 µg/Lane)
Lane 8: Rat liver tissue lysate (30 µg/Lane)
Lane 9: Mouse brain tissue lysate (30 µg/Lane)
Lane 10: Rat brain tissue lysate (30 µg/Lane)
Lane 11: Mouse pancreas tissue lysate (30 µg/Lane)
Predicted band size: 61 kDa
Observed band size: 65 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721379) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"