UBE2T Recombinant Rabbit Monoclonal Antibody [JE65-79]
Catalog# HA721385
UBE2T Recombinant Rabbit Monoclonal Antibody [JE65-79]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
UBE2T Recombinant Rabbit Monoclonal Antibody [JE65-79]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human UBE2T aa 1-100 / 197.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 22.5 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, A549 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, SK-OV-3 cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HeLa.
Conjugation
unconjugated
Clone Number
JE65-79
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
Background References
1. Zhu J et al. UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma. J Transl Med. 2021 Aug
2. Zhu Z et al. UBE2T-mediated Akt ubiquitination and Akt/β-catenin activation promotes hepatocellular carcinoma development by increasing pyrimidine metabolism. Cell Death Dis. 2022 Feb
Subcellular Location
Nucleus.
Synonyms
Cell proliferation inducing gene 50 protein antibody
Cell proliferation-inducing gene 50 protein antibody
HSPC150 antibody
HSPC150 protein similar to ubiquitin conjugating enzyme antibody
PIG50 antibody
Ube2t antibody
UBE2T_HUMAN antibody
Ubiquitin carrier protein T antibody
Ubiquitin conjugating enzyme antibody
ubiquitin conjugating enzyme E2 T antibody
ExpandImages
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Western blot analysis of UBE2T on different lysates with Rabbit anti-UBE2T antibody (HA721385) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: K-562 cell lysate (15 µg/Lane)
Lane 3: MCF7 cell lysate (15 µg/Lane)
Lane 4: A549 cell lysate (15 µg/Lane)
Lane 5: HEK-293 cell lysate (15 µg/Lane)
Lane 6: Jurkat cell lysate (15 µg/Lane)
Lane 7: SK-OV-3 cell lysate (15 µg/Lane)
Lane 8: SH-SY5Y cell lysate (15 µg/Lane)
Lane 9: HepG2 cell lysate (15 µg/Lane)
Lane 10: Mouse testis tissue lysate (30 µg/Lane)
Lane 11: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 22.5 kDa
Observed band size: 22.5 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721385) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling UBE2T with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"