EXOC2 Recombinant Rabbit Monoclonal Antibody [JE65-98]
Catalog# HA721386
EXOC2 Recombinant Rabbit Monoclonal Antibody [JE65-98]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
EXOC2 Recombinant Rabbit Monoclonal Antibody [JE65-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human EXOC2 aa 1-100 / 924.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 104 kDa
Positive Control
K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, HeLa cell lysate, HEK-293 cell lysate, U-2 OS cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE65-98
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
Target
Function
Exocyst complex component 2 is a protein that in humans is encoded by the EXOC2 gene. The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts.
Background References
1. Van Bergen NJ et al. Mutations in the exocyst component EXOC2 cause severe defects in human brain development. J Exp Med. 2020 Oct
2. Saternus R et al. A closer look at evolution: Variants (SNPs) of genes involved in skin pigmentation, including EXOC2, TYR, TYRP1, and DCT, are associated with 25(OH)D serum concentration. Endocrinology. 2015 Jan
Subcellular Location
Midbody, Midbody ring.
Synonyms
EXOC2 antibody
EXOC2_HUMAN antibody
Exocyst complex component 2 antibody
Exocyst complex component Sec5 antibody
FLJ11026 antibody
SEC5 antibody
SEC5 like 1 antibody
SEC5L1 antibody
Sec5p antibody
Images
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Western blot analysis of EXOC2 on different lysates with Rabbit anti-EXOC2 antibody (HA721386) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: MCF7 cell lysate (15 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (15 µg/Lane)
Lane 6: HeLa cell lysate (15 µg/Lane)
Lane 7: HEK-293 cell lysate (15 µg/Lane)
Lane 8: U-2 OS cell lysate (15 µg/Lane)
Lane 9: Human brain tissue lysate (30 µg/Lane)
Lane 10: Mouse brain tissue lysate (30 µg/Lane)
Lane 11: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721386) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"