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RAB22A Recombinant Rabbit Monoclonal Antibody [JE66-08]

Usd: 385

Specification

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Catalog# HA721387

RAB22A Recombinant Rabbit Monoclonal Antibody [JE66-08]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RAB22A Recombinant Rabbit Monoclonal Antibody [JE66-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human RAB22A aa 1-100 / 194.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 22 kDa

Positive Control

HepG2 cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, Jurkat cell lysate, U-2 OS cell lysate, A549 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, Rat testis lysate, MCF7.

Conjugation

unconjugated

Clone Number

JE66-08

RRID

AB_3072504

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell
human
Tested Tested
Not Recommended
Not Recommended
Tested Tested
mouse
Tested Tested
Not Recommended
 
 
rat
Tested Tested
Not Recommended
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Ras-related protein Rab-22A is a protein that in humans is encoded by the RAB22A gene. The protein encoded by this gene is a member of the RAB family of small GTPases. The GTP-bound form of the encoded protein has been shown to interact with early-endosomal antigen 1, and may be involved in the trafficking of and interaction between endosomal compartments.

Background References

1. Gao Y et al. Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity. Cell Res. 2022 Dec

2. Liao D et al. Chromosomal translocation-derived aberrant Rab22a drives metastasis of osteosarcoma. Nat Cell Biol. 2020 Jul

Subcellular Location

Endosome membrane, Cell membrane, Early endosome, Late endosome, Cell projection, ruffle, Cytoplasmic vesicle, Cytoplasmic vesicle, phagosome, phagosome membrane.

UNIPROT

SwissProt: Q9UL26 Human

SwissProt: P35285 Mouse

Entrez Gene: 366265 Rat

Synonyms

3732413A17Rik antibody

AI662177 antibody

AW319644 antibody

AW550514 antibody

E130120E14Rik antibody

GTP binding protein RAB22A antibody

MGC16770 antibody

OTTMUSP00000017605 antibody

Rab 22 antibody

RAB 22A antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (<a href="/products/HA721387" style="font-weight: bold;text-decoration: underline;">HA721387</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate (10 µg/Lane)<br />Lane 2: HAP1-RAB22A KD cell lysate (10 µg/Lane)<br /><br />Predicted band size: 22 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 120 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721387" style="font-weight: bold;text-decoration: underline;">HA721387</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate (10 µg/Lane)
    Lane 2: HAP1-RAB22A KD cell lysate (10 µg/Lane)

    Predicted band size: 22 kDa
    Observed band size: 20 kDa

    Exposure time: 120 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (<a href="/products/HA721387" style="font-weight: bold;text-decoration: underline;">HA721387</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (15 µg/Lane)<br />Lane 2: K-562 cell lysate (15 µg/Lane)<br />Lane 3: HeLa cell lysate (15 µg/Lane)<br />Lane 4: MCF7 cell lysate (15 µg/Lane)<br />Lane 5: Jurkat cell lysate (15 µg/Lane)<br />Lane 6: U-2 OS cell lysate (15 µg/Lane)<br />Lane 7: A549 cell lysate (15 µg/Lane)<br />Lane 8: HEK-293 cell lysate (15 µg/Lane)<br />Lane 9: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 10: Rat brain tissue lysate (30 µg/Lane)<br />Lane 11: Mouse kidney tissue lysate (30 µg/Lane)<br />Lane 12: Mouse testis tissue lysate (30 µg/Lane)<br />Lane 13: Rat testis lysate (30 µg/Lane)<br /><br />Predicted band size: 22 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 5 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721387" style="font-weight: bold;text-decoration: underline;">HA721387</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (15 µg/Lane)
    Lane 2: K-562 cell lysate (15 µg/Lane)
    Lane 3: HeLa cell lysate (15 µg/Lane)
    Lane 4: MCF7 cell lysate (15 µg/Lane)
    Lane 5: Jurkat cell lysate (15 µg/Lane)
    Lane 6: U-2 OS cell lysate (15 µg/Lane)
    Lane 7: A549 cell lysate (15 µg/Lane)
    Lane 8: HEK-293 cell lysate (15 µg/Lane)
    Lane 9: Mouse brain tissue lysate (30 µg/Lane)
    Lane 10: Rat brain tissue lysate (30 µg/Lane)
    Lane 11: Mouse kidney tissue lysate (30 µg/Lane)
    Lane 12: Mouse testis tissue lysate (30 µg/Lane)
    Lane 13: Rat testis lysate (30 µg/Lane)

    Predicted band size: 22 kDa
    Observed band size: 20 kDa

    Exposure time: 5 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of MCF7 cells labeling RAB22A with Rabbit anti-RAB22A antibody (<a href="/products/HA721387" style="font-weight: bold;text-decoration: underline;">HA721387</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB22A antibody (<a href="/products/HA721387" style="font-weight: bold;text-decoration: underline;">HA721387</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of MCF7 cells labeling RAB22A with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"