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ESE1 Recombinant Rabbit Monoclonal Antibody [JE33-30]

Usd: 385

Specification

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Catalog# HA721388

ESE1 Recombinant Rabbit Monoclonal Antibody [JE33-30]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ESE1 Recombinant Rabbit Monoclonal Antibody [JE33-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human ESE1 aa 1-371 / 371.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 41.5 kDa

Positive Control

A549 cell lysate, SK-Br-3 cell lysate, A431 cell lysate, mouse lung tissue lysate, rat kidney tissue lysate, A431.

Conjugation

unconjugated

Clone Number

JE33-30

RRID

AB_3072505

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

EARLY FLOWERING 3 (ELF3) is a plant-specific gene that encodes the hydroxyproline-rich glycoprotein and is required for the function of the circadian clock. ELF3 is one of the three components that make up the Evening Complex (EC) within the plant circadian clock, in which all three components reach peak gene expression and protein levels at dusk. ELF3 serves as a scaffold to bind EARLY FLOWERING 4 (ELF4) and LUX ARRHYTHMO (LUX), two other components of the EC, and functions to control photoperiod sensitivity in plants. ELF3 also plays an important role in temperature and light input within plants for circadian clock entrainment. Additionally, it plays roles in light and temperature signaling that are independent from its role in the EC.

Background References

1. Jung JH et al. A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis. Nature. 2020 Sep

2. Wang J et al. High glucose mediates NLRP3 inflammasome activation via upregulation of ELF3 expression. Cell Death Dis. 2020 May

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P78545 Human

SwissProt: Q3UPW2 Mouse

SwissProt: Q4V7E1 Rat

Synonyms

E74 like factor 3 antibody

E74 like factor 3 ets domain transcription factor antibody

E74 like factor 3 ets domain transcription factor epithelial specific antibody

E74 like factor 3 ETS domain transcription factor serine box epithelial specific antibody

E74-like factor 3 antibody

Elf3 antibody

ELF3_HUMAN antibody

Epithelial restricted with serine box antibody

Epithelial-restricted with serine box antibody

Epithelium restricted Ets protein ESX antibody

Expand

Images

  • Western blot analysis of ESE1 on different lysates with Rabbit anti-ESE1 antibody (<a href="/products/HA721388" style="font-weight: bold;text-decoration: underline;">HA721388</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (10 µg/Lane)<br />Lane 2: SK-Br-3 cell lysate (10 µg/Lane)<br />Lane 3: A431 cell lysate (10 µg/Lane)<br />Lane 4: Mouse lung tissue lysate (20 µg/Lane)<br />Lane 5: Rat kidney tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 41.5 kDa<br />Observed band size: 41.5 kDa<br /><br />Exposure time: 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721388" style="font-weight: bold;text-decoration: underline;">HA721388</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ESE1 on different lysates with Rabbit anti-ESE1 antibody (HA721388) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (10 µg/Lane)
    Lane 2: SK-Br-3 cell lysate (10 µg/Lane)
    Lane 3: A431 cell lysate (10 µg/Lane)
    Lane 4: Mouse lung tissue lysate (20 µg/Lane)
    Lane 5: Rat kidney tissue lysate (20 µg/Lane)

    Predicted band size: 41.5 kDa
    Observed band size: 41.5 kDa

    Exposure time: 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of A431 cells labeling ESE1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721388" style="font-weight: bold;text-decoration: underline;">HA721388</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling ESE1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721388, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"