HRPT2 Recombinant Rabbit Monoclonal Antibody [JE66-42]
Catalog# HA721390
HRPT2 Recombinant Rabbit Monoclonal Antibody [JE66-42]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
HRPT2 Recombinant Rabbit Monoclonal Antibody [JE66-42]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human HRPT2 aa 331-430 / 531.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 61 kDa
Positive Control
SW480 cell lysate, K-562 cell lysate, HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, MCF7 cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, human kidney tissue, human testis tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
JE66-42
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:200
Target
Function
Cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae), also known as CDC73 and parafibromin, is a protein which in humans is encoded by the CDC73 gene. Parafibromin, LEO1, PAF1, and CTR9 form the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. Mutations in the CDC73 gene are associated with hyperparathyroidism-jaw tumor syndrome (HPT-JT) and parathyroid carcinomas.
Background References
1. Radzikowski KC et al. 18F Choline PET/CT in a patient with HRPT2 mutation: Detecting parathyroid carcinoma recurrence and concomitant breast carcinoma. Nuklearmedizin. 2022 Feb
2. Kim SY et al. USP37 Deubiquitinates CDC73 in HPT-JT Syndrome. Int J Mol Sci. 2022 Jun
Subcellular Location
Nucleus.
Synonyms
C1orf28 antibody
CDC 73 antibody
Cdc73 antibody
CDC73_HUMAN antibody
Cell division cycle 73 antibody
Cell division cycle 73 Paf1/RNA polymerase II complex component homolog antibody
Cell division cycle 73 Paf1/RNA polymerase II complex component like protein antibody
Cell division cycle protein 73 homolog antibody
FIHP antibody
FLJ23316 antibody
ExpandImages
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Western blot analysis of HRPT2 on different lysates with Rabbit anti-HRPT2 antibody (HA721390) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: RAW264.7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721390) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of HRPT2 on different lysates with Rabbit anti-HRPT2 antibody (HA721390) at 1/1,000 dilution.
Lane 1: SW480 cell lysate
Lane 2: K-562 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: MCF7 cell lysate
Lane 7: HEK-293 cell lysate
Lane 8: RAW264.7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721390) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HRPT2 antibody (HA721390) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721390) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-HRPT2 antibody (HA721390) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721390) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-HRPT2 antibody (HA721390) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721390) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"