HEC1 Recombinant Rabbit Monoclonal Antibody [JE31-10]
Catalog# HA721395
HEC1 Recombinant Rabbit Monoclonal Antibody [JE31-10]
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WB
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Human
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unconjugated
Overview
Product Name
HEC1 Recombinant Rabbit Monoclonal Antibody [JE31-10]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HEC1 aa 593-642 / 642.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 74 kDa
Positive Control
K-562 cell lysate, U-2 OS cell lysate, HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, HepG2 cell lysate, LO2 cell lysate.
Conjugation
unconjugated
Clone Number
JE31-10
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene. Ndc80 is one of the proteins of outer kinetochore. It forms a heterotetramer with proteins NUF2, SPC25, and SPC24. This protein complex has microtubule-binding domains. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved. NDC80 has been shown to interact with MIS12, NEK2 and PSMC2.v
Background References
1. Wimbish RT et al. Hec1/Ndc80 Tail Domain Function at the Kinetochore-Microtubule Interface. Front Cell Dev Biol. 2020 Feb
2. Iemura K et al. Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelity. J Cell Biol. 2021 Jul
Subcellular Location
Nucleus, Chromosome, centromere, kinetochore.
UNIPROT
Synonyms
from Entrez Gene
antibody
HEC antibody
Highly expressed in cancer antibody
Highly expressed in cancer protein antibody
Highly expressed in cancer rich in leucine heptad repeats antibody
HsHec1 antibody
hsNDC80 antibody
Kinetochore associated 2 antibody
Kinetochore associated protein 2 antibody
ExpandImages
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Western blot analysis of HEC1 on different lysates with Rabbit anti-HEC1 antibody (HA721395) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: HeLa cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: HepG2 cell lysate
Lane 8: LO2 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721395) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"