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Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]

Usd: 385

Specification

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Catalog# HA721396

Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Alpha 1 Acid Glycoprotein aa 51-100 / 201.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 23.5 kDa

Positive Control

Human small intestine tissue lysates, human liver tissue.

Conjugation

unconjugated

Clone Number

JE31-16

RRID

AB_3072513

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Alpha-1-acid glycoprotein 1 is a protein that in humans is encoded by the ORM1 gene. This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. ORM1 has been shown to interact with Plasminogen activator inhibitor-1.

Background References

1. Joyce KE et al. Postexercise urinary alpha-1 acid glycoprotein is not dependent on hypoxia. J Appl Physiol (1985). 2022 Jan

2. Durmuş M et al. Does alpha-1-acid glycoprotein determine for infertility in polycystic ovary syndrome? Eur J Obstet Gynecol Reprod Biol. 2022 Jul

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02763 Human

Synonyms

A1AG1_HUMAN antibody

AGP 1 antibody

AGP A antibody

AGP antibody

AGP1 antibody

Alpha 1 acid glycoprotein antibody

Alpha-1-acid glycoprotein 1 antibody

alpha-1-AGP antibody

Epididymis secretory sperm binding protein Li 153w antibody

glycoprotein, alpha-1-acid, of serum antibody

Expand

Images

  • Western blot analysis of Alpha 1 Acid Glycoprotein on human small intestine tissue lysates with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (<a href="/products/HA721396" style="font-weight: bold;text-decoration: underline;">HA721396</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 23.5 kDa<br />Observed band size: 45 kDa (Glycosylation)<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721396" style="font-weight: bold;text-decoration: underline;">HA721396</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Alpha 1 Acid Glycoprotein on human small intestine tissue lysates with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/1,000 dilution.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 23.5 kDa
    Observed band size: 45 kDa (Glycosylation)

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721396) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (<a href="/products/HA721396" style="font-weight: bold;text-decoration: underline;">HA721396</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721396" style="font-weight: bold;text-decoration: underline;">HA721396</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721396) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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