Ran Recombinant Rabbit Monoclonal Antibody [JE33-28]
Catalog# HA721406
Ran Recombinant Rabbit Monoclonal Antibody [JE33-28]
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Ran Recombinant Rabbit Monoclonal Antibody [JE33-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Ran aa 167-216 / 216.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, LoVo cell lysate, Jurkat cell lysate, MDA-MB-231 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, rat thymus tissue lysate, HeLa.
Conjugation
unconjugated
Clone Number
JE33-28
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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FC
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1:500-1:1,000
Target
Function
Ran (RAs-related Nuclear protein) also known as GTP-binding nuclear protein Ran is a protein that in humans is encoded by the RAN gene. Ran is a small 25 kDa protein that is involved in transport into and out of the cell nucleus during interphase and also involved in mitosis. It is a member of the Ras superfamily. Ran is a small G protein that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein has also been implicated in the control of DNA synthesis and cell cycle progression, as mutations in Ran have been found to disrupt DNA synthesis.
Background References
1. Boudhraa Z et al. Ran GTPase: A Key Player in Tumor Progression and Metastasis. Front Cell Dev Biol. 2020 May
2. Guo S et al. RAN proteins in neurodegenerative disease: Repeating themes and unifying therapeutic strategies. Curr Opin Neurobiol. 2022 Feb
Subcellular Location
Nucleus, Nucleus envelope, Cytoplasm, cytosol, Melanosome.
Synonyms
Androgen receptor associated protein 24 antibody
Androgen receptor-associated protein 24 antibody
ARA 24 antibody
ARA24 antibody
Gsp1 antibody
GTP binding nuclear protein RAN antibody
GTP-binding nuclear protein Ran antibody
GTPase Ran antibody
Guanosine triphosphatase Ran antibody
LPS antibody
ExpandImages
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Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (HA721406) at 1/1,000 dilution.
Lane 1: A549 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: LoVo cell lysate (15 µg/Lane)
Lane 5: Jurkat cell lysate (15 µg/Lane)
Lane 6: MDA-MB-231 cell lysate (15 µg/Lane)
Lane 7: MCF7 cell lysate (15 µg/Lane)
Lane 8: RAW264.7 cell lysate (15 µg/Lane)
Lane 9: NIH/3T3 cell lysate (15 µg/Lane)
Lane 10: Rat thymus tissue lysate (30 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721406) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of HeLa cells labeling Ran.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721406, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"