ROR2 Recombinant Rabbit Monoclonal Antibody [JE37-72]
Catalog# HA721417
ROR2 Recombinant Rabbit Monoclonal Antibody [JE37-72]
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WB
-
IHC-P
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Human
-
unconjugated
Overview
Product Name
ROR2 Recombinant Rabbit Monoclonal Antibody [JE37-72]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ROR2 aa 34-83 / 943.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 105 kDa
Positive Control
K-562 cell lysate, U-2 OS cell lysate, HEK-293 cell lysate, Jurkat cell lysate, human placenta tissue.
Conjugation
unconjugated
Clone Number
JE37-72
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Tyrosine-protein kinase transmembrane receptor ROR2, also known as neurotrophic tyrosine kinase, receptor-related 2, is a protein that in humans is encoded by the ROR2 gene located on position 9 of the long arm of chromosome 9. This protein is responsible for aspects of bone and cartilage growth. It is involved in Robinow syndrome and autosomal dominant brachydactyly type B. ROR2 is a member of the receptor tyrosine kinase-like orphan receptor (ROR) family. The protein encoded by this gene is a receptor tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development.
Background References
1. Thorup AS et al. ROR2 blockade as a therapy for osteoarthritis. Sci Transl Med. 2020 Sep
2. Liu D et al. ROR1 and ROR2 expression in pancreatic cancer. BMC Cancer. 2021 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
BDB antibody
BDB1 antibody
Brachydactyly type B antibody
EC 2.7.10.1 antibody
MGC163394 antibody
Neurotrophic tyrosine kinase antibody
Neurotrophic tyrosine kinase, receptor related 2 antibody
NTRKR2 antibody
Receptor tyrosine kinase-like orphan receptor 2 antibody
receptor-related 2 antibody
ExpandImages
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Western blot analysis of ROR2 on different lysates with Rabbit anti-ROR2 antibody (HA721417) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721417) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ROR2 antibody (HA721417) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721417) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"