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ROR2 Recombinant Rabbit Monoclonal Antibody [JE37-72]

Usd: 385

Specification

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Catalog# HA721417

ROR2 Recombinant Rabbit Monoclonal Antibody [JE37-72]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ROR2 Recombinant Rabbit Monoclonal Antibody [JE37-72]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ROR2 aa 34-83 / 943.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 105 kDa

Positive Control

K-562 cell lysate, U-2 OS cell lysate, HEK-293 cell lysate, Jurkat cell lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

JE37-72

RRID

AB_3072534

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Tyrosine-protein kinase transmembrane receptor ROR2, also known as neurotrophic tyrosine kinase, receptor-related 2, is a protein that in humans is encoded by the ROR2 gene located on position 9 of the long arm of chromosome 9. This protein is responsible for aspects of bone and cartilage growth. It is involved in Robinow syndrome and autosomal dominant brachydactyly type B. ROR2 is a member of the receptor tyrosine kinase-like orphan receptor (ROR) family. The protein encoded by this gene is a receptor tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development.

Background References

1. Thorup AS et al. ROR2 blockade as a therapy for osteoarthritis. Sci Transl Med. 2020 Sep

2. Liu D et al. ROR1 and ROR2 expression in pancreatic cancer. BMC Cancer. 2021 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q01974 Human

Synonyms

BDB antibody

BDB1 antibody

Brachydactyly type B antibody

EC 2.7.10.1 antibody

MGC163394 antibody

Neurotrophic tyrosine kinase antibody

Neurotrophic tyrosine kinase, receptor related 2 antibody

NTRKR2 antibody

Receptor tyrosine kinase-like orphan receptor 2 antibody

receptor-related 2 antibody

Expand

Images

  • Western blot analysis of ROR2 on different lysates with Rabbit anti-ROR2 antibody (<a href="/products/HA721417" style="font-weight: bold;text-decoration: underline;">HA721417</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: U-2 OS cell lysate<br />Lane 3: HEK-293 cell lysate<br />Lane 4: Jurkat cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 105 kDa<br />Observed band size: 105 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721417" style="font-weight: bold;text-decoration: underline;">HA721417</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ROR2 on different lysates with Rabbit anti-ROR2 antibody (HA721417) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: U-2 OS cell lysate
    Lane 3: HEK-293 cell lysate
    Lane 4: Jurkat cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 105 kDa
    Observed band size: 105 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721417) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ROR2 antibody (<a href="/products/HA721417" style="font-weight: bold;text-decoration: underline;">HA721417</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721417" style="font-weight: bold;text-decoration: underline;">HA721417</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ROR2 antibody (HA721417) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721417) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"