Desmocollin 1 Recombinant Rabbit Monoclonal Antibody [JE66-57]
Catalog# HA721418
Desmocollin 1 Recombinant Rabbit Monoclonal Antibody [JE66-57]
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WB
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Mouse
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Rat
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unconjugated
Overview
Product Name
Desmocollin 1 Recombinant Rabbit Monoclonal Antibody [JE66-57]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Desmocollin 1 aa 795-894 / 894.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 100 kDa
Positive Control
C2C12 cell lysate, L6 cell lysate, RH-35 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, mouse heart tissue lysate, rat heart tissue lysate.
Conjugation
unconjugated
Clone Number
JE66-57
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Desmocollin-1 is a protein that in humans is encoded by the DSC1 gene. The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Alternative splicing results in two transcript variants encoding distinct isoforms. DSC1 has been shown to interact with Desmoglein 2.
Background References
1. Qie X et al. Role of DSC1 in Drosophila melanogaster synaptic activities in response to haedoxan A. Insect Sci. 2023 Feb
2. Zhang Z et al. CircRAB11FIP1 promoted autophagy flux of ovarian cancer through DSC1 and miR-129. Cell Death Dis. 2021 Feb
Subcellular Location
Cell membrane, Cell junction, desmosome.
Synonyms
Cadherin family member 1 antibody
CDHF1 antibody
Desmocollin1 antibody
Desmosomal glycoprotein 2/3 antibody
DG2/DG3 antibody
DSC 1 antibody
DSC1 antibody
Images
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Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (HA721418) at 1/1,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: L6 cell lysate
Lane 3: RH-35 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Desmocollin 1 on different lysates with Rabbit anti-Desmocollin 1 antibody (HA721418) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Mouse heart tissue lysate
Lane 4: Rat heart tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721418) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"