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IGFBP7 Recombinant Rabbit Monoclonal Antibody [JE34-01]

Usd: 385

Specification

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Catalog# HA721423

IGFBP7 Recombinant Rabbit Monoclonal Antibody [JE34-01]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

IGFBP7 Recombinant Rabbit Monoclonal Antibody [JE34-01]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human IGFBP7 aa 51-250 / 282.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, FC

Molecular Weight

Predicted band size: 29 kDa

Positive Control

HeLa cell lysate, SK-OV-3 cell lysate, SW480 cell lysate, 293T cell lysate, MCF7 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat pancreas tissue, HeLa.

Conjugation

unconjugated

Clone Number

JE34-01

RRID

AB_3072540

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:100

  • FC

  • 1:500-1:1,000

Target

Function

Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with low affinity compared to IGFBPs 1-6. It also stimulates cell adhesion. The protein is implicated in some cancers. IGFBP7 has been shown to interact with Insulin-like growth factor 1, VPS24, and the IGF-1 receptor (IGF1R).

Background References

1. Ko T et al. Cardiac fibroblasts regulate the development of heart failure via Htra3-TGF-β-IGFBP7 axis. Nat Commun. 2022 Jun

2. Zhao Q et al. Increased IGFBP7 Expression Correlates with Poor Prognosis and Immune Infiltration in Gastric Cancer. J Cancer. 2021 Jan

Subcellular Location

Secreted.

UNIPROT

SwissProt: Q16270 Human

SwissProt: Q61581 Mouse

Entrez Gene: 289560 Rat

Synonyms

AGM antibody

Angiomodulin antibody

FSTL2 antibody

IBP-7 antibody

IBP7 antibody

IBP7_HUMAN antibody

IGF binding protein 7 antibody

IGF-binding protein 7 antibody

IGFBP rP1 antibody

IGFBP-7 antibody

Expand

Images

  • Western blot analysis of IGFBP7 on different lysates with Rabbit anti-IGFBP7 antibody (<a href="/products/HA721423" style="font-weight: bold;text-decoration: underline;">HA721423</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (15 µg/Lane)<br />Lane 2: SK-OV-3 cell lysate (15 µg/Lane)<br />Lane 3: SW480 cell lysate (15 µg/Lane)<br />Lane 4: 293T cell lysate (15 µg/Lane)<br />Lane 5: MCF7 cell lysate (15 µg/Lane)<br />Lane 6: Mouse kidney tissue lysate (30 µg/Lane)<br />Lane 7: Rat kidney tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29 kDa<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721423" style="font-weight: bold;text-decoration: underline;">HA721423</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IGFBP7 on different lysates with Rabbit anti-IGFBP7 antibody (HA721423) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (15 µg/Lane)
    Lane 2: SK-OV-3 cell lysate (15 µg/Lane)
    Lane 3: SW480 cell lysate (15 µg/Lane)
    Lane 4: 293T cell lysate (15 µg/Lane)
    Lane 5: MCF7 cell lysate (15 µg/Lane)
    Lane 6: Mouse kidney tissue lysate (30 µg/Lane)
    Lane 7: Rat kidney tissue lysate (30 µg/Lane)

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-IGFBP7 antibody (<a href="/products/HA721423" style="font-weight: bold;text-decoration: underline;">HA721423</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721423" style="font-weight: bold;text-decoration: underline;">HA721423</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-IGFBP7 antibody (HA721423) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721423) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of HeLa cells labeling IGFBP7 with Rabbit anti-IGFBP7 antibody (<a href="/products/HA721423" style="font-weight: bold;text-decoration: underline;">HA721423</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IGFBP7 antibody (<a href="/products/HA721423" style="font-weight: bold;text-decoration: underline;">HA721423</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling IGFBP7 with Rabbit anti-IGFBP7 antibody (HA721423) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IGFBP7 antibody (HA721423) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of HeLa cells labeling IGFBP7.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721423" style="font-weight: bold;text-decoration: underline;">HA721423</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling IGFBP7.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721423, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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