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PAR2 Recombinant Rabbit Monoclonal Antibody [JE35-05]

Usd: 385

Specification

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Catalog# HA721426

PAR2 Recombinant Rabbit Monoclonal Antibody [JE35-05]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

PAR2 Recombinant Rabbit Monoclonal Antibody [JE35-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PAR2 aa 328-377 / 397.

Species Reactivity

Human, Mouse

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 44 kDa

Positive Control

HCT 116 cell lysate, HepG2 cell lysate, PANC-1 cell lysate, PC-3M cell lysate, AGS cell lysate, SGC-7901 cell lysate, human kidney tissue lysate, human stomach tissue lysate, mouse large intestine tissue lysate, HepG2.

Conjugation

unconjugated

Clone Number

JE35-05

RRID

AB_3072543

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

Protease activated receptor 2 (PAR2) also known as coagulation factor II (thrombin) receptor-like 1 (F2RL1) or G-protein coupled receptor 11 (GPR11) is a protein that in humans is encoded by the F2RL1 gene. PAR2 modulates inflammatory responses, obesity, metabolism, cancers and acts as a sensor for proteolytic enzymes generated during infection. In humans, we can find PAR2 in the stratum granulosum layer of epidermal keratinocytes. Functional PAR2 is also expressed by several immune cells such as eosinophils, neutrophils, monocytes, macrophages, dendritic cells, mast cells and T cells.

Background References

1. Zhao J et al. PAR2 Mediates Itch via TRPV3 Signaling in Keratinocytes. J Invest Dermatol. 2020 Aug

2. Suhaj P et al. PAR2: The Cornerstone of Pancreatic Diseases. Physiol Res. 2022 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P55085 Human

SwissProt: P55086 Mouse

Synonyms

Coagulation factor II receptor like 1 antibody

Coagulation factor II receptor-like 1 antibody

Coagulation factor II thrombin receptor like 1 antibody

F2RL1 antibody

G protein coupled receptor 11 antibody

G-protein coupled receptor 11 antibody

GPR11 antibody

PAR 2 antibody

PAR-2 antibody

PAR2_HUMAN antibody

Expand

Images

  • Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (<a href="/products/HA721426" style="font-weight: bold;text-decoration: underline;">HA721426</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate (15 µg/Lane)<br />Lane 2: HepG2 cell lysate (15 µg/Lane)<br />Lane 3: PANC-1 cell lysate (15 µg/Lane)<br />Lane 4: PC-3M cell lysate (15 µg/Lane)<br />Lane 5: AGS cell lysate (15 µg/Lane)<br />Lane 6: SGC-7901 cell lysate (15 µg/Lane)<br />Lane 7: Human kidney tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 44 kDa<br />Observed band size: 55 kDa (Glycosylation)<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721426" style="font-weight: bold;text-decoration: underline;">HA721426</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (HA721426) at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate (15 µg/Lane)
    Lane 2: HepG2 cell lysate (15 µg/Lane)
    Lane 3: PANC-1 cell lysate (15 µg/Lane)
    Lane 4: PC-3M cell lysate (15 µg/Lane)
    Lane 5: AGS cell lysate (15 µg/Lane)
    Lane 6: SGC-7901 cell lysate (15 µg/Lane)
    Lane 7: Human kidney tissue lysate (30 µg/Lane)

    Predicted band size: 44 kDa
    Observed band size: 55 kDa (Glycosylation)

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721426) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HepG2 cells labeling PAR2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721426" style="font-weight: bold;text-decoration: underline;">HA721426</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling PAR2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721426, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"