IGFBP1 Recombinant Rabbit Monoclonal Antibody [JE35-39]
Catalog# HA721429
IGFBP1 Recombinant Rabbit Monoclonal Antibody [JE35-39]
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WB
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Human
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unconjugated
Overview
Product Name
IGFBP1 Recombinant Rabbit Monoclonal Antibody [JE35-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IGFBP1 aa 1-259/259.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 28 kDa
Positive Control
Human placenta tissue lysate, human liver tissue lysate.
Conjugation
unconjugated
Clone Number
JE35-39
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Insulin-like growth factor-binding protein 1 (IBP-1) also known as placental protein 12 (PP12) is a protein that in humans is encoded by the IGFBP1 gene. This gene is a member of the Insulin-like growth factor-binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a type-I thyroglobulin domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Background References
1. Hivert MF et al. Placental IGFBP1 levels during early pregnancy and the risk of insulin resistance and gestational diabetes. Med. 2024 Jun
2. Cai G et al. IGFBP1 Sustains Cell Survival during Spatially-Confined Migration and Promotes Tumor Metastasis. Adv Sci (Weinh). 2023 Jul
Subcellular Location
Secreted.
UNIPROT
Synonyms
AFBP antibody
Alpha pregnancy associated endometrial globulin antibody
Amniotic fluid binding protein antibody
Binding protein 25 antibody
Binding protein 26 antibody
Binding protein 28 antibody
Growth hormone independent binding protein antibody
hIGFBP 1 antibody
hIGFBP1 antibody
IBP 1 antibody
ExpandImages
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Western blot analysis of IGFBP1 on different lysates with Rabbit anti-IGFBP1 antibody (HA721429) at 1/1,000 dilution.
Lane 1: Human placenta tissue lysate
Lane 2: Human liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 28 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721429) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"