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FRS2 Recombinant Rabbit Monoclonal Antibody [JE30-77]

Usd: 385

Specification

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Catalog# HA721434

FRS2 Recombinant Rabbit Monoclonal Antibody [JE30-77]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

FRS2 Recombinant Rabbit Monoclonal Antibody [JE30-77]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human FRS2 aa 91-240 / 508.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 57 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, HEK-293 cell lysate, NCI-H441 cell lysate, MCF7 cell lysate, HeLa.

Conjugation

unconjugated

Clone Number

JE30-77

RRID

AB_3072550

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

Fibroblast growth factor receptor substrate 2 is a protein that in humans is encoded by the FRS2 gene. FRS2 is an 80 kDa membrane-anchored signal transducing adaptor protein (STAP) that links specific activated Receptor Tyrosine Kinases (RTKs) to multiple downstream signaling pathways, most notably the MAPK/ERK, PI3K/AKT/mTOR and PLCγ pathways. It is overexpressed and amplified in several cancer types, including prostate cancer.

Background References

1. Jing W et al. Expression of FRS2 in atypical lipomatous tumor/well-differentiated liposarcoma and dedifferentiated liposarcoma: an immunohistochemical analysis of 182 cases with genetic data. Diagn Pathol. 2021 Oct

2. Clark JF et al. FRS2-independent GRB2 interaction with FGFR2 is not required for embryonic development. bioRxiv. 2023 Mar

Subcellular Location

Endomembrane system.

UNIPROT

SwissProt: Q8WU20 Human

Synonyms

FGFR signaling adaptor SNT antibody

FGFR signalling adaptor antibody

FGFR substrate 2 antibody

FGFR-signaling adaptor SNT antibody

Fibroblast growth factor receptor substrate 2 antibody

FRS 2 antibody

FRS2 antibody

FRS2_HUMAN antibody

FRS2A antibody

FRS2alpha antibody

Expand

Images

  • Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody (<a href="/products/HA721434" style="font-weight: bold;text-decoration: underline;">HA721434</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: HEK-293 cell lysate<br />Lane 4: NCI-H441 cell lysate<br />Lane 5: MCF7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721434" style="font-weight: bold;text-decoration: underline;">HA721434</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody (HA721434) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HEK-293 cell lysate
    Lane 4: NCI-H441 cell lysate
    Lane 5: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 70 kDa

    Exposure time: 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721434) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling FRS2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721434" style="font-weight: bold;text-decoration: underline;">HA721434</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling FRS2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721434, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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