Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56]
Catalog# HA721440
Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56]
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WB
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Human
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unconjugated
Overview
Product Name
Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Palladin aa 1-100 / 1,383.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 151 kDa
Positive Control
HeLa cell lysate, SiHa cell lysate, A431 cell lysate, SW480 cell lysate, 293T cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate.
Conjugation
unconjugated
Clone Number
JE66-56
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Palladin is a protein that in humans is encoded by the PALLD gene. Palladin is a component of actin-containing microfilaments that control cell shape, adhesion, and contraction. It is a part of the myotilin-myopalladin-palladin family and may play an important role in modulating the actin cytoskeleton. Palladin, in contrast to myotilin and myopalladin, which are expressed only in striated muscle, is expressed ubiquitously in cells of mesenchymal origin. Palladin's precise biological role is poorly understood, but it has been shown to play a role in cytoskeletal organization, embryonic development, cell motility, scar formation in the skin, and nerve cell development.
Background References
1. Mayer O et al. Cytoskeletal Protein Palladin in Adult Gliomas Predicts Disease Incidence, Progression, and Prognosis. Cancers (Basel). 2022 Oct
2. Shu X et al. Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling. Cancer Med. 2023 Feb
Subcellular Location
Cytoplasm, cytoskeleton, Cytoplasm, myofibril, sarcomere, Z line.
UNIPROT
Synonyms
CGI 151 antibody
CGI151 antibody
KIAA0992 antibody
MYN antibody
Myoneurin antibody
pa antibody
Palladin cytoskeletal associated protein antibody
Palld antibody
PNCA1 antibody
Sarcoma antigen NY SAR 77 antibody
ExpandImages
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Western blot analysis of Palladin on different lysates with Rabbit anti-Palladin antibody (HA721440) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15µg/Lane)
Lane 2: SiHa cell lysate (10µg/Lane)
Lane 3: A431 cell lysate (15 µg/Lane)
Lane 4: SW480 cell lysate (30 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 8: MDA-MB-468 cell lysate (20 µg/Lane)
Lane 9: MCF7 cell lysate (20 µg/Lane)
Predicted band size: 151 kDa
Observed band size: 110/151 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721440) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"