Phospho-Vimentin (S39) Recombinant Rabbit Monoclonal Antibody [JE43-26] (HA721442)
Catalog# HA721442
Rabbit Monoclonal to Phospho-Vimentin (S38)
Tested for
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WB
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IF-Cell
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Vimentin (S39) on different lysates with Rabbit anti-Phospho-Vimentin (S39) antibody (HA721442) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721442) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Vimentin (S39) on different lysates with Rabbit anti-Phospho-Vimentin (S39) antibody (HA721442) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721442) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.