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ATG10 Recombinant Rabbit Monoclonal Antibody [JE59-86]

Usd: 385

Specification

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Catalog# HA721444

ATG10 Recombinant Rabbit Monoclonal Antibody [JE59-86]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ATG10 Recombinant Rabbit Monoclonal Antibody [JE59-86]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide corresponding to Human ATG10 aa 180-220 (C terminal)

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 25 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, K-562 cell lysate.

Conjugation

unconjugated

Clone Number

JE59-86

RRID

AB_3072560

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Autophagy-related protein 10 is a protein that in humans is encoded by the ATG10 gene. E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.

Background References

1. Sun W et al. The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. Theranostics. 2020 Jan

2. Castro I et al. Functional Genetic Variants in ATG10 Are Associated with Acute Myeloid Leukemia. Cancers (Basel). 2021 Mar

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9H0Y0 Human

Synonyms

Apg 10 antibody

APG 10L antibody

APG10 antibody

APG10 autophagy 10 like antibody

APG10 like antibody

APG10, S. cerevisiae, homolog of antibody

APG10-like antibody

APG10L antibody

ATG 10 antibody

ATG10 antibody

Expand

Images

  • Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (<a href="/products/HA721444" style="font-weight: bold;text-decoration: underline;">HA721444</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: K-562 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721444" style="font-weight: bold;text-decoration: underline;">HA721444</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (HA721444) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K-562 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 30 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"