CABP1 Recombinant Rabbit Monoclonal Antibody [JE56-72]
Catalog# HA721448
CABP1 Recombinant Rabbit Monoclonal Antibody [JE56-72]
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WB
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FC
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Human
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unconjugated
Overview
Product Name
CABP1 Recombinant Rabbit Monoclonal Antibody [JE56-72]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CABP aa 150-250.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
U-87 MG cell lysate, human brain tissue lysate, U-87 MG.
Conjugation
unconjugated
Clone Number
JE56-72
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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FC
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1:500-1:1,000
Target
Function
Calcium binding protein 1 is a protein that in humans is encoded by the CABP1 gene. Calcium-binding protein 1 is a calcium-binding protein discovered in 1999. It has two EF hand motifs and is expressed in neuronal cells in such areas as hippocampus, habenular nucleus of the epithalamus, Purkinje cell layer of the cerebellum, and the amacrine cells and cone bipolar cells of the retina. Calcium-binding protein 1 which is a neuron -specific member of the calmodulin (CaM) superfamily which modulates Ca2+-dependent activity of inositol trisphosphate receptors (InsP3RS). L-CaBP1 is also associated with the cytoskeleton structures. But the S-CaBP1 is situated in or near the plasma membrane. In brain, CaBp1 is found in the cerebral cortex and hippocampus and in the protein, Cabp1 is found in cone bipolar and amacrine cells. We can also express that CaBP1 may regulate Ca2+ dependent activity of InSP3Rs by promoting structural contacts between suppressor and core domains but has no effect on INsP3 binding to the receptor. CaBP1 contains four EF-hands in two separate domains namely, EF1 and Ef2 is contained in N-domain whereas Ef3 and EF4 is contained in c domain to which Ca2+ binds.[7] Calcium-binding protein 1 (CaBP1) is placed in the lumen of the endoplasmic reticulum.it is relocated outside cells during apoptosis and involved in the phagocytosis of apoptotic cells. CaBP1 and CaM. lobes fold independently. CaBP1-CaM chimeras based on exchange of three elements these are N-lobe, C-lobe and inter lobe linker. Expression of CaBP1 helps to block Ca2+-dependent facilitation of P/Q-type Ca2+ current which is markedly reduced facilitation of synaptic transmission.
Background References
1. Ames JB. L-Type Ca2+ Channel Regulation by Calmodulin and CaBP1. Biomolecules. 2021 Dec
2. Salveson I et al. Chemical shift assignments of the C-terminal domain of CaBP1 bound to the IQ-motif of voltage-gated Ca2+ channel (CaV1.2). Biomol NMR Assign. 2022 Oct
Subcellular Location
Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Synapse.
UNIPROT
Synonyms
CABP 1 antibody
CaBP1 antibody
CABP1_HUMAN antibody
Calbrain antibody
Calcium binding protein 1 antibody
Calcium binding protein 5 antibody
Calcium-binding protein 1 antibody
Caldendrin antibody
HCALB BR antibody
HCALB_BR antibody
Images
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Western blot analysis of CABP1 on different lysates with Rabbit anti-CABP1 antibody (HA721448) at 1/1,000 dilution.
Lane 1: U-87 MG cell lysate (20 µg/Lane)
Lane 2: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721448) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of U-87 MG cells labeling CABP1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721448, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"