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SHB Recombinant Rabbit Monoclonal Antibody [JE57-12]

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Specification

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Catalog# HA721449

SHB Recombinant Rabbit Monoclonal Antibody [JE57-12]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

SHB Recombinant Rabbit Monoclonal Antibody [JE57-12]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SHB aa 450-509.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 55 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, K-562 cell lysate, HEK-293 cell lysate, A549.

Conjugation

unconjugated

Clone Number

JE57-12

RRID

AB_3072565

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.

Background References

1. He Q et al. Pericyte dysfunction due to Shb gene deficiency increases B16F10 melanoma lung metastasis. Int J Cancer. 2020 Nov

2. Jamalpour M et al. Absence of the Shb gene in mixed-lineage leukemia MLL-AF9 cells increases latency in mice despite higher proliferation rates in vitro. Exp Cell Res. 2020 Dec

Subcellular Location

Cytoplasm, Cell membrane.

UNIPROT

SwissProt: Q15464 Human

Synonyms

bA3J10.2 antibody

OTTHUMP00000021397 antibody

RP11 3J10.8 antibody

RP113J10.8 antibody

SH2 domain containing adapter protein B antibody

SH2 domain-containing adapter protein B antibody

SHB (Src homology 2 domain containing) adaptor protein B antibody

SHB adaptor protein (a Src homology 2 protein) antibody

SHB adaptor protein antibody

Shb antibody

Expand

Images

  • Western blot analysis of SHB on different lysates with Rabbit anti-SHB antibody (<a href="/products/HA721449" style="font-weight: bold;text-decoration: underline;">HA721449</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: HEK-293 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 35 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721449" style="font-weight: bold;text-decoration: underline;">HA721449</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SHB on different lysates with Rabbit anti-SHB antibody (HA721449) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: HEK-293 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

    Exposure time: 35 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721449) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of A549 cells labeling SHB.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721449" style="font-weight: bold;text-decoration: underline;">HA721449</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A549 cells labeling SHB.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721449, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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