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Cytokeratin 5+6 Recombinant Rabbit Monoclonal Antibody [PSH0-67]

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Specification

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Catalog# HA721455

Cytokeratin 5+6 Recombinant Rabbit Monoclonal Antibody [PSH0-67]

Application

  • IHC-P

  • IF-Tissue

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cytokeratin 5+6 Recombinant Rabbit Monoclonal Antibody [PSH0-67]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Cytokeratin 6 186-235.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, IF-Tissue, WB

Molecular Weight

Predicted band size: 60 kDa

Positive Control

Human breast tissue, mouse skin tissue, rat skin tissue, mouse brain tissue lysate, rat brain tissue lysate, human lung squamous cell carcinoma tissue, human skin tissue, human tonsil tissue.

Conjugation

unconjugated

Clone Number

PSH0-67

RRID

AB_3072571

Reactivity Data

IHC-P IF-Tissue WB
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200-1:1,000

  • IF-Tissue

  • 1:1,000

  • WB

  • 1:1,000

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies.

Background References

1. Yamamoto Y et al. Cytokeratin 5/6 expression in pT1 bladder cancer predicts intravesical recurrence in patients treated with bacillus Calmette-Guérin instillation. Pathology. 2022 Oct

2. Iakymenko OA et al. Utility of D2-40, Cytokeratin 5/6, and High-Molecular-weight Cytokeratin (Clone 34βE12) in Distinguishing Intraductal Spread of Urothelial Carcinoma From Prostatic Stromal Invasion. Am J Surg Pathol. 2022 Apr

Subcellular Location

Cytoskeleton.

UNIPROT

SwissProt: P02538 Human

SwissProt: P04259 Human

SwissProt: P13647 Human

Synonyms

58 kDa cytokeratin antibody

CK-5 antibody

CK5 antibody

Cytokeratin-5 antibody

Cytokeratin5 antibody

DDD antibody

DDD1 antibody

EBS2 antibody

epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody

K2C5_HUMAN antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Cytokeratin 5+6 on different lysates with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse skin tissue lysate (hot lysis)<br />Lane 2: Rat skin tissue lysate (hot lysis)<br />Lane 3: Rat skin tissue lysate (RIPA lysis)<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 60 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cytokeratin 5+6 on different lysates with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    Lane 1: Mouse skin tissue lysate (hot lysis)
    Lane 2: Rat skin tissue lysate (hot lysis)
    Lane 3: Rat skin tissue lysate (RIPA lysis)

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721455) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling Cytokeratin 5+6 with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling Cytokeratin 5+6 with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721455, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5+6 with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5+6 with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721455, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Cytokeratin 5+6 antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721455" style="font-weight: bold;text-decoration: underline;">HA721455</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Cytokeratin 5+6 antibody (HA721455) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721455) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin embedded human lung squamous cell carcinoma tissue using anti-Cytokeratin 5+6 antibody (1/1,000) performed on the Leica&reg; BOND&trade; RX.

    Immunohistochemical analysis of paraffin embedded human lung squamous cell carcinoma tissue using anti-Cytokeratin 5+6 antibody (1/1,000) performed on the Leica® BOND™ RX.

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Citation