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LSD2 Recombinant Rabbit Monoclonal Antibody [JE32-40]

Usd: 385

Specification

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Catalog# HA721462

LSD2 Recombinant Rabbit Monoclonal Antibody [JE32-40]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

LSD2 Recombinant Rabbit Monoclonal Antibody [JE32-40]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human LSD2 aa 71-120 / 822.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 92 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, HCT 116 cell lysate.

Conjugation

unconjugated

Clone Number

JE32-40

RRID

AB_3072578

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Lysine (K)-specific demethylase 1B is a protein that in humans is encoded by the KDM1B gene. Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function. Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes.

Background References

1. Costa DFD et al. Transcriptional deregulation of LSD1 and LSD2 is associated with cytogenetic risk in chronic lymphocytic leukemia. Leuk Lymphoma. 2022 Dec

2. Niwa H et al. Structure-Activity Relationship and In Silico Evaluation of cis- and trans-PCPA-Derived Inhibitors of LSD1 and LSD2. ACS Med Chem Lett. 2022 Aug

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: Q8NB78 Human

Synonyms

amine oxidase (flavin containing) domain 1 antibody

amine oxidase, flavin containing 1 antibody

AOF1 antibody

bA204B7.3 antibody

C6orf193 antibody

dJ298J15.2 antibody

Flavin-containing amine oxidase domain-containing protein 1 antibody

FLJ33898 antibody

FLJ34109 antibody

FLJ43328 antibody

Expand

Images

  • Western blot analysis of LSD2 on different lysates with Rabbit anti-LSD2 antibody (<a href="/products/HA721462" style="font-weight: bold;text-decoration: underline;">HA721462</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: MCF7 cell lysate<br />Lane 6: U-2 OS cell lysate<br />Lane 7: HCT 116 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 92 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721462" style="font-weight: bold;text-decoration: underline;">HA721462</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LSD2 on different lysates with Rabbit anti-LSD2 antibody (HA721462) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: A549 cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: MCF7 cell lysate
    Lane 6: U-2 OS cell lysate
    Lane 7: HCT 116 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 92 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721462) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of LSD2 on different lysates with Rabbit anti-LSD2 antibody (<a href="/products/HA721462" style="font-weight: bold;text-decoration: underline;">HA721462</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si LSD2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 92 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721462" style="font-weight: bold;text-decoration: underline;">HA721462</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of LSD2 on different lysates with Rabbit anti-LSD2 antibody (HA721462) at 1/2,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si LSD2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 92 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721462) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"